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WH-4-023 | Inhibitor of LCK, SRC
RATINGS:
Cellular Use: (5 reviews)

In Model Organisms: (3 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
LCK
  • IC 50:0.6 nM
  • IC 50:61 nM
SRC
  • IC 50:1 nM
  • IC 50:61 nM
Inhibitor
--

Selectivity

In Vitro Selectivity Assessment

Potency: IC50 - BTK 0.1 uM, FGFR10.11 uM, JAK2 0.24 uM, KDR 0.14 uM, p38 alpha 0.53 uM, SYK 0.2 uM, TIE2 0.2 uM, ZAP70 0.37 uM

Potency
Cellular
In Vitro

LCK

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/jm060435i

SRC

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? No

DOI Reference: 10.1021/jm060435i

In Vivo Validations

Rat
Dose: 5 mg/kg
Route of delivery: Oral
Organ of interest (O): T cell

DOI Reference: 10.1021/jm060435i

Orthogonal Probes def

A-770041

Chemical Information

Molecular Formula C32H36N6O4
SMILEs COc1ccc(N(C(=O)Oc2c(C)cccc2C)c2ccnc(Nc3ccc(N4CCN(C)CC4)cc3)n2)c(OC)c1
InChI InChI=1S/C32H36N6O4/c1-22-7-6-8-23(2)30(22)42-32(39)38(27-14-13-26(40-4)21-28(27)41-5)29-15-16-33-31(35-29)34-24-9-11-25(12-10-24)37-19-17-36(3)18-20-37/h6-16,21H,17-20H2,1-5H3,(H,33,34,35)
Molecular weight 568.28 Da
AlogP 5.94314
HBond acceptors 10
HBond donors 1
Atoms 78
PAINS * Yes

* This is an automated alert only, and may not necessarily indicate an issue with this probe. ( Learn more about PAINS )

References

Publications

Cross References

canSARChEMBLBindingDB

Vendors

Note: This is not an exhaustive list and does not indicate endorsement by the portal.

Expert Reviews

by SERP
(on 14 Jun 2016 )
Cellular Use Rating
In Model Organisms
( The reviewer did not leave any comments )
by SERP
(on 15 Jun 2016 )
Cellular Use Rating
This is a very promiscuous tyrosine kinase inhibitor.
by SERP
(on 23 Jun 2016 )
Cellular Use Rating
WH-4-023 is compound 28 in the referenced work. However, this compound is one of a number synthesised, and the actual paper discusses detailed profiling of a compound 43. This later compound is more...
by SERP
(on 27 Jun 2016 )
Cellular Use Rating
In Model Organisms
This appears to be non-selective versus Src. Further kinase profiling is needed prior to recommendation as a probe for Lck.
by SERP
(on 29 Jun 2016 )
Cellular Use Rating
In Model Organisms
Poor bioavailability hampers use of WH-4-023 as in vivo probe in mice.
Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria