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WEE1-IN-7 | WEE1-IN-7 : Inhibitor of WEE1
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (2 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
WEE1
  • IC50:2.1 ± 0.3 nM
  • IC50:82 ± 2 nM
  • IC50:430 ±32 nM
Inhibitor
up to 1 uM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
Kinase profiling was performed at a concentration of 1 μM against 225 kinase targets and found that ...

Potency
Cellular
In Vitro

WEE1

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acs.jmedchem.3c02434

In Vivo Validations

Mouse
Dose: 1 mg/Kg
Route of delivery: Intravenous
Plasma half life: 1.06 h
Systemic clearance: 8.2 L/h/Kg
Area Under the Curve:: 118 h·ng/mL
Volume of Distribution at Steady-State: 11.3 L/Kg

DOI Reference: 10.1021/acs.jmedchem.3c02434

Dose: 60 mg/Kg
Route of delivery: Intragastric
Plasma half life: 5.94 h
Cmax: 498
Area Under the Curve:: 4342 h·ng/mL
Bioavailability: 61.46%

DOI Reference: 10.1021/acs.jmedchem.3c02434

Chemical Information

Molecular Formula C28H28N10O
SMILEs C=CCn1c(=O)c2cnc(Nc3ccc(N4CCN(C)CC4)cc3)nc2n1-c1cccc(-c2cnccn2)n1
InChI InChI=1S/C28H28N10O/c1-3-13-37-27(39)22-18-31-28(32-20-7-9-21(10-8-20)36-16-14-35(2)15-17-36)34-26(22)38(37)25-6-4-5-23(33-25)24-19-29-11-12-30-24/h3-12,18-19H,1,13-17H2,2H3,(H,31,32,34)
Molecular weight 520.24 Da
AlogP 3.1156000000000015
HBond acceptors 11
HBond donors 1
Atoms 67
PAINS * Yes

* This is an automated alert only, and may not necessarily indicate an issue with this probe. ( Learn more about PAINS )

Vendors

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Expert Reviews

by SERP
(on 17 Aug 2024 )
Cellular Use Rating
In Model Organisms
This appears to be a slightly more selective Wee1 inhibitor (in biochemical assays) than the other one listed in the portal - the different chemotype suggests that perhaps using both in an orthogonal manner...
by SERP
(on 11 Nov 2024 )
Cellular Use Rating
In Model Organisms
Wee1-IN-12h is currently one of the best available tool compounds for the WEE1 protein kinase. It is a potent, ATP-competitive inhibitor, exhibiting a Kd of 2.1 nM. It has been developed primarily for...
Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria