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VinSpinIn | VinSpinIn : Inhibitor of SPIN1, SPIN2A, SPIN2B, SPIN3, SPIN4
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (0 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
SPIN1
  • Kd, IC50:9.9 nM. (Kd); 33 nM (IC50)
  • ΔTm:13.17
  • EC50:270 nM
SPIN2A
      SPIN2B
      • ΔTm:10.47
        SPIN3
        • ΔTm:14.12
          SPIN4
          • ΔTm:6.53
            Inhibitor
            0.5 - 3 uM

            Selectivity

            In Vitro Selectivity Assessment
            Potency Assay Off-Target:
            ITC, DSF
            Selectivity Assessment Description:
            Selectivity was assessed by ITC on four SPIN subfamily members. SPIN1-49–262 (9.9 nM), SPIN1-26–2 ...

            Potency
            Cellular
            In Vitro

            SPIN1

            Mode of Action: Inhibitor

            Structure-Activity-Relationship data available? Yes

            DOI Reference: 10.1021/acs.jmedchem.9b00562

            SPIN2A

            Mode of Action: Inhibitor

            Structure-Activity-Relationship data available? No

            DOI Reference: 10.1021/acs.jmedchem.9b00562

            SPIN2B

            Mode of Action: Inhibitor

            Structure-Activity-Relationship data available? No

            DOI Reference: 10.1021/acs.jmedchem.9b00562

            SPIN3

            Mode of Action: Inhibitor

            Structure-Activity-Relationship data available? No

            DOI Reference: 10.1021/acs.jmedchem.9b00562

            SPIN4

            Mode of Action: Inhibitor

            Structure-Activity-Relationship data available? No

            DOI Reference: 10.1021/acs.jmedchem.9b00562

            Negative Control Compounds

            canSAR3148937
            Notes: VinSpinInC was tested in Thermal Shift Assay against the Spin family members providing no changes in thermal stability.

            Orthogonal Probes def

            EML631

            Chemical Information

            Molecular Formula C42H58N8O4
            SMILEs CC1(C)C(N)=Nc2cc(OCCCN3Cc4ccc(OCCc5cn(CC(=O)N6CCC(CCN7CCCC7)CC6)nn5)cc4C3)c(OCC3CC3)cc21
            InChI InChI=1S/C42H58N8O4/c1-42(2)36-23-38(54-29-31-6-7-31)39(24-37(36)44-41(42)43)53-20-5-16-48-25-32-8-9-35(22-33(32)26-48)52-21-13-34-27-50(46-45-34)28-40(51)49-18-11-30(12-19-49)10-17-47-14-3-4-15-47/h8-9,22-24,27,30-31H,3-7,10-21,25-26,28-29H2,1-2H3,(H2,43,44)
            Molecular weight 738.46 Da
            AlogP 5.4775000000000045
            HBond acceptors 12
            HBond donors 2
            Atoms 112

            Vendors

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            Expert Reviews

            by SERP
            (on 20 Mar 2021 )
            Cellular Use Rating
            This compound is quite specific to the Spin family proteins, therefore it will be helpful for studying cellular functions of the family. Especially the control compound with similar structure and same...
            by SERP+
            (on 23 Sept 2024 )
            Cellular Use Rating
            This is a well characterised compound, but both the active and inactive compounds displayed toxicity, implying off-target effects. Despite significant effort, the toxicity issues relating to this series...
            Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria