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THNAN69 | THNAN69 : Degrader (PROTAC) of LIMK2
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (0 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
LIMK2
  • Kd:249 nM
  • EC50:80 nM
  • DC50:1 nM
Degrader (PROTAC)
10-100 nM

Selectivity

In Cell Selectivity Assessment
Potency Assay Off-Target:
Selectivity against LIMK1 assessed via NanoBRET EC50 412 nM
In Cell Selectivity Assessment
Potency Assay Off-Target:
Full cellular (MOLT4) proteomics shows no significant degradation of other targets.

Potency
Cellular
In Vitro

LIMK2

Mode of Action: Degrader (PROTAC)

Structure-Activity-Relationship data available? No

Other Reference: Peer Reviewed at SGC

Negative Control Compounds

canSAR7437556
Notes: THNAN69-NC: No LIMK2 degradation: Tested at 1 µM in western blot and at up to 10µM in EGFP-LIMK2 assay. DCmax at 10nM = 0% (vs DMSO).

Chemical Information

Molecular Formula C32H29Cl2F2N9O4S
SMILEs Cc1c(OCc2cn(CCCCC(=O)Nc3ncc(-c4cc(C(F)F)nn4-c4c(Cl)cccc4Cl)s3)nn2)cccc1N1CCC(=O)NC1=O
InChI InChI=1S/C32H29Cl2F2N9O4S/c1-18-23(44-13-11-28(47)39-32(44)48)8-5-9-25(18)49-17-19-16-43(42-40-19)12-3-2-10-27(46)38-31-37-15-26(50-31)24-14-22(30(35)36)41-45(24)29-20(33)6-4-7-21(29)34/h4-9,14-16,30H,2-3,10-13,17H2,1H3,(H,37,38,46)(H,39,47,48)
Molecular weight 743.14 Da
AlogP 6.974420000000006
HBond acceptors 13
HBond donors 2
Atoms 79

References

Publications

Cross References

https://www.thesgc.org/node/15729

Expert Reviews

by SERP
(on 6 Mar 2026 )
Cellular Use Rating
THNAN69 is a selective degrader of LIMK2 over LIMK1. The PROTAC binds to both LIMK1 and LIMK2 (demonstrated by NanoBRET), but only degrades LIMK2 with a DC50 of 1 nM and a Dmax of 88% at 10 nM - a small...
by SERP
(on 8 Mar 2026 )
Cellular Use Rating
THNAN69 is suitable for use as a probe in cellular assays. Its potential for in vivo applications remains to be determined pending additional data. With a DC50 of 1 nM and Dmax values of 88.2% at 10 nM...
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