SGC-PI5P4Kg/MYLK-1 | SGC-PI5P4Kg/MYLK-1 : Inhibitor of PIP4K2C, MYLK4
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (0 reviews)
In Vivo
Vendors

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
PIP4K2C
  • Kd:19 nM
  • IC50:67 nM
MYLK4
  • Kd:12 nM
  • IC50:14 nM
Inhibitor
500 nM
up to 1 uM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
SGC-PI5P4Kγ/MYLK4-1 was profiled in the DiscoverX scanMAX assay against 403 wild-type kinases at 1 μ ...
In Cell Selectivity Assessment
Potency Assay Off-Target:
NanoBRET analysis of cellular binding affinity for PIKfyve (NB IC50 ​= ​450 ​nM), CLK2 (NB IC50 ​= ​ ...

Potency
Cellular
In Vitro

PIP4K2C

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1016/j.crchbi.2022.100036

MYLK4

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1016/j.crchbi.2022.100036

Negative Control Compounds

SGC-PI5P4Kγ/MYLK4-1N
Notes: cpd 4 shows enzymatic Kd 6400 nM and NanoBRET IC50 >10000 nM
SMILES: NC1=NC2=C(CCCCC3=C2C4=C(C=CC(C#CC5CC5)=C4)N3)C=N1

Chemical Information

Molecular Formula C20H20N4O
SMILEs Nc1ncc2c(n1)-c1c([nH]c3ccc(C4=CCOCC4)cc13)CCC2
InChI InChI=1S/C20H20N4O/c21-20-22-11-14-2-1-3-17-18(19(14)24-20)15-10-13(4-5-16(15)23-17)12-6-8-25-9-7-12/h4-6,10-11,23H,1-3,7-9H2,(H2,21,22,24)
Molecular weight 332.16 Da
AlogP 0.0
HBond acceptors 5
HBond donors 3
Atoms 45

Expert Reviews


(on 12 Jun 2023 )
Cellular Use Rating
Good in vitro probe. It is important to use the negative control probe in parallel as it allows to account for the off target activity on PYKfyve. The cellular engagement IC50 of 67nM was obtained in the...
(on 17 Jun 2024 )
Cellular Use Rating
This probe was thoroughly characterized. Target engagement was demonstrated by different orthogonal assays. A commercial kinase panel was used to investigate selectivity. A negative-control is available....
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