SGC-CLK-1 | SGC-CLK-1 : ATP Competitive inhibitor of CLK1, CLK2 and CLK4
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (1 reviews)
In Vivo
Vendors

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
CLK1
  • IC50:13 nM
  • IC50:165 nM
CLK2
  • IC50:4 nM
  • IC50:100 nM
CLK4
  • IC50:46 nM
  • IC50:70 nM
ATP competitive
up to 1 uM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
KINOMEscan
Selectivity Assessment Description:
Only 6/403 kinases with PoC<35 when screened at 1 µM in KINOMEscan. (ERK8, NEK7, PIP5K2B, HIPK1 50 n ...

Potency
Cellular
In Vitro

CLK1

Mode of Action: ATP competitive

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.3390/ijms21207549

CLK2

Mode of Action: ATP competitive

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.3390/ijms21207549

CLK4

Mode of Action: ATP competitive

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.3390/ijms21207549

Negative Control Compounds

SGC-CLK-1
Notes: SGC-CLK-1N was tested in the Discoverx panel and no kinases had PoC<45. The negative control was sent to Eurofins for testing in enzyme assays for CLKs and typical CLK inhibitor off targets, showing no inhibition.

Chemical Information

Molecular Formula C19H15F3N6O2
SMILEs COc1cc(Nc2nccc(-c3cnn4nc(OC)ccc34)n2)cc(C(F)(F)F)c1
InChI InChI=1S/C19H15F3N6O2/c1-29-13-8-11(19(20,21)22)7-12(9-13)25-18-23-6-5-15(26-18)14-10-24-28-16(14)3-4-17(27-28)30-2/h3-10H,1-2H3,(H,23,25,26)
Molecular weight 416.12 Da
AlogP 3.965900000000002
HBond acceptors 8
HBond donors 1
Atoms 45

References

Cross References

SGC Chemical Probes

Expert Reviews


(on 6 May 2021 )
Cellular Use Rating
This probe has potent activity against the CLK family of CMGC kinases (1,2,4). It seems to be a good probe for these targets in cells. However, there is no data provided for the most related kinases,...
(on 27 May 2021 )
Cellular Use Rating
In Model Organisms
( The reviewer did not leave any comments )
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