SGC-CDKL5_GSK3 | SGC-CDKL5_GSK3 : ATP competitive, Inhibitor of CDKL5, GSK3A, GSK3B
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (0 reviews)
In Vivo
Vendors

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
CDKL5
  • IC50:6.5 nM
  • IC50:3.5 nM
GSK3A
  • IC50:4.0 nM
  • IC50:10 nM
GSK3B
  • IC50:9.0
  • IC50:35 nM
up to 500 nM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
Compounds 2 and 4, were analysed at 1 μM in the Eurofins DiscoverX scanMAX panel, assessing binding ...
In Cell Selectivity Assessment
Potency Assay Off-Target:
NanoBRET assay for closest off-target CDK16 and related CDK17: IC50 of 1.9 μM for bot targets

Potency
Cellular
In Vitro

CDKL5

Mode of Action: ATP competitive

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acschemneuro.3c00135

GSK3A

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acschemneuro.3c00135

GSK3B

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acschemneuro.3c00135

Negative Control Compounds

CANSAR3935430
Notes: cpd 4 was 400-fold less active (IC50 = 1400 nM) in NanoBRET, and IC50 840 nM in biochemical assay

Chemical Information

Molecular Formula C16H17F2N5O2
SMILEs O=C(Nc1cn[nH]c1C(=O)NC1CCNCC1)c1cc(F)cc(F)c1
InChI InChI=1S/C16H17F2N5O2/c17-10-5-9(6-11(18)7-10)15(24)22-13-8-20-23-14(13)16(25)21-12-1-3-19-4-2-12/h5-8,12,19H,1-4H2,(H,20,23)(H,21,25)(H,22,24)
Molecular weight 349.14 Da
AlogP 0.0
HBond acceptors 7
HBond donors 4
Atoms 42

Expert Reviews


(on 17 Jun 2024 )
Cellular Use Rating
The probe is highly potent in cellular and cell free settings, was assessed thoroughly and screened against a panel of 402 kinases (Eurofins DiscoverX scanMAX) without any additional off targets. A control...
(on 23 Sept 2024 )
Cellular Use Rating
( The reviewer did not leave any comments )
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