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RO2468 | Antagonist of MDM2
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (2 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
MDM2
  • IC50:6 nM
  • IC50:0.015 uM
Antagonist
up to 1 uM

Selectivity

Potency
Cellular
In Vitro

MDM2

Mode of Action: Antagonist

Structure-Activity-Relationship data available? No

In Vivo Validations

Male C57 mice, Female nude mice
Dose: 10 mg/Kg QD
Route of delivery: Intravenous, Oral
Plasma half life: 3 h (IV)
Systemic clearance: 1,8 mL/min/kg (IV)
Organ of interest (O): SJSA-1 xenografts (nude mice, 3-20 mg/kg)

Reference: --

Orthogonal Probes def

AM-6761
MI-77301
AMG232

Chemical Information

Molecular Formula C30H30Cl2FN5O4
SMILEs COc1cc(C(N)=O)ccc1NC(=O)[C@@H]1N[C@@H](CC(C)(C)C)[C@@]2(C(=O)Nc3cc(Cl)ncc32)[C@H]1c1cccc(Cl)c1F
InChI InChI=1S/C30H30Cl2FN5O4/c1-29(2,3)12-21-30(16-13-35-22(32)11-19(16)37-28(30)41)23(15-6-5-7-17(31)24(15)33)25(38-21)27(40)36-18-9-8-14(26(34)39)10-20(18)42-4/h5-11,13,21,23,25,38H,12H2,1-4H3,(H2,34,39)(H,36,40)(H,37,41)/t21-,23-,25+,30+/m0/s1
Molecular weight 613.17 Da
AlogP 5.024
HBond acceptors 9
HBond donors 5
Atoms 72

References

Publications

    Cross References

    canSARChEMBLBindingDB

    Vendors

    Note: This is not an exhaustive list and does not indicate endorsement by the portal.

    Expert Reviews

    by SERP
    (on 29 Apr 2020 )
    Cellular Use Rating
    In Model Organisms
    This is an excellent chemical probe and although the selectivity data is lacking, the biological effect in p53 WT vs MUT cell lines looks convincing (over 700 fold difference in IC50s). Still, I would...
    by SERP
    (on 16 May 2020 )
    Cellular Use Rating
    In Model Organisms
    This is overall an excellent chemical probe against MDM2, based on in vitro biochemical binding affinity, cellular activity and selectivity, and an impressive in vivo/xenografts...
    Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria