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PT2385 | Antagonist of EPAS1
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (3 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

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Targets Biochemical/Biophysical Potency Cellular Potency
EPAS1
  • Kd:< 50 nM
  • IC50:12 ± 5 nM
  • Kd:167 nM
  • IC50:27 ± 6 nM
  • IC50:42-44 nM
Antagonist
up to 1 uM

Selectivity

In Vitro Selectivity Assessment

Potency: HIF-1alpha - not detected

Potency Assay Off-Target:
ITC
In Cell Selectivity Assessment
Selectivity Assessment Description:
0/40 kinase selectivity (&lt;70% activity remaining at 10 μM)

Potency
Cellular
In Vitro

EPAS1

Mode of Action: Antagonist

Structure-Activity-Relationship data available? No

DOI Reference: 10.1021/acs.jmedchem.8b01196

In Vivo Validations

Mouse, Rat, Dog
Dose: 3 mg/Kg (IV), 10 mg/Kg (PO)
Route of delivery: Intravenous, Oral
Plasma half life: 3.3 h (M, R), 11 h (Dog)
Systemic clearance: 19.9 (M), 34.6 (R), 21.5. (D) mL/min/kg

DOI Reference: 10.1021/acs.jmedchem.8b01196

Negative Control Compounds

9a

Orthogonal Probes def

M1001
T1001
PT2399

Chemical Information

Molecular Formula C17H12F3NO4S
SMILEs CS(=O)(=O)c1ccc(Oc2cc(F)cc(C#N)c2)c2c1[C@H](O)C(F)(F)C2
InChI InChI=1S/C17H12F3NO4S/c1-26(23,24)14-3-2-13(12-7-17(19,20)16(22)15(12)14)25-11-5-9(8-21)4-10(18)6-11/h2-6,16,22H,7H2,1H3/t16-/m0/s1
Molecular weight 383.04 Da
AlogP 3.117980000000002
HBond acceptors 5
HBond donors 1
Atoms 38

Vendors

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Expert Reviews

by SERP
(on 20 May 2021)
Cellular Use Rating
In Model Organisms
There are multiple lines of convincing evidence to support meaningful HIF2α modulation by PT-2385 in cell-free, cellular, and in vivo assays. This includes nanomolar cell-free target inhibition, x-ray...
by SERP
(on 4 Jun 2021)
Cellular Use Rating
In Model Organisms
(The reviewer did not leave any public comments)
by SERP
(on 4 Jun 2021)
Cellular Use Rating
In Model Organisms
This is a promising HiF-2a antagonist with a high affinity to target, high cell potency, expected cellular effects (in vitro and in vivo), good in vivo PK and toxicology profiles, and very good selectivity...
Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria