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PT-65 | PT-65 : Degrader (PROTAC) of GSK3A, GSK3B
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (0 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
GSK3A
    • DC50:28.3 nM
    • Dmax:94.3 ± 2.2%
    GSK3B
    • Kd:12.41 nM
    • DC50:34.2 nM
    • Dmax:90.8 ± 2.9%
    Degrader (PROTAC)
    up to 500 nM

    Selectivity

    In Cell Selectivity Assessment
    Potency Assay Off-Target:
    The selectivity of PT-65 was evaluated by the global proteome analysis using tandem mass tag (TMT)-b ...

    Potency
    Cellular
    In Vitro

    GSK3A

    Mode of Action: Degrader (PROTAC)

    Structure-Activity-Relationship data available? Yes

    DOI Reference: 10.1016/j.ejmech.2021.113889

    GSK3B

    Mode of Action: Degrader (PROTAC)

    Structure-Activity-Relationship data available? Yes

    DOI Reference: 10.1016/j.ejmech.2021.113889

    Chemical Information

    Molecular Formula C51H63N13O12S
    SMILEs Nc1ncc(-c2ccc(S(=O)(=O)N3CCN(CCCCc4cn(CCOCCOCCOCCOCCOCCNc5cccc6c5C(=O)N(C5CCC(=O)NC5=O)C6=O)nn4)CC3)cc2)nc1C(=O)Nc1cccnc1
    InChI InChI=1S/C51H63N13O12S/c52-47-46(49(67)56-37-6-4-15-53-33-37)57-42(34-55-47)36-9-11-39(12-10-36)77(70,71)63-20-18-61(19-21-63)17-2-1-5-38-35-62(60-59-38)22-24-73-26-28-75-30-32-76-31-29-74-27-25-72-23-16-54-41-8-3-7-40-45(41)51(69)64(50(40)68)43-13-14-44(65)58-48(43)66/h3-4,6-12,15,33-35,43,54H,1-2,5,13-14,16-32H2,(H2,52,55)(H,56,67)(H,58,65,66)
    Molecular weight 1081.44 Da
    AlogP 0.0
    HBond acceptors 25
    HBond donors 5
    Atoms 140

    References

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    Cross References

    canSARChEMBLBindingDB

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    Expert Reviews

    by SERP
    (on 18 May 2023 )
    Cellular Use Rating
    The proteomics for this compound was done in singlicate (and the raw data is not available), so there is no P-values/fold change information available. I, therefore, think it will not be possible to adequately...
    by SERP
    (on 19 May 2023 )
    Cellular Use Rating
    This molecule can potently degrade with GSK3a and GSK3b with a relatively rapid degradation profile at 1uM in SHSY5Y cells. Adequate controls are provided to demonstrate proteasomal dependence and competition...
    Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria