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PFI-5 | PFI-5 : inhibitor of SMYD2
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (0 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
SMYD2
  • IC50:16 nM
  • IC50:8 nM
  • EC50:130 nM
mixed interaction mode
1-3 uM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
Within target family: Biochemical selectivity across a wide number of SAM-dependent methyltransferas ...
In Cell Selectivity Assessment
Potency Assay Off-Target:
PFI-5 was investigated in a cellular H4K20me1 methylation assay in MCF7 cells and no significant eff ...

Potency
Cellular
In Vitro

SMYD2

Mode of Action: mixed interaction mode

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acs.jmedchem.9b00112

Negative Control Compounds

CANSAR3139360
Notes: PFI-5N shows no effect in cells (IC50 >25 uM in enzymatic assay)

Orthogonal Probes def

BAY-598

Chemical Information

Molecular Formula C29H39N7O
SMILEs Cc1cc2c(ncn2C2(C)CN(C(=O)c3ccn(CC4CCN([C@H]5C=CCCCC5)CC4)c3)C2)c(N)n1
InChI InChI=1S/C29H39N7O/c1-21-15-25-26(27(30)32-21)31-20-36(25)29(2)18-35(19-29)28(37)23-11-12-33(17-23)16-22-9-13-34(14-10-22)24-7-5-3-4-6-8-24/h5,7,11-12,15,17,20,22,24H,3-4,6,8-10,13-14,16,18-19H2,1-2H3,(H2,30,32)/t24-/m0/s1
Molecular weight 501.32 Da
AlogP 4.205420000000003
HBond acceptors 8
HBond donors 2
Atoms 76

Expert Reviews

by SERP
(on 21 Feb 2023 )
Cellular Use Rating
PFI-5 is a quality chemical probe with a good negative control and together with BAY-598 has the cellular biology of SMYD2 covered. There is clearly sufficient data to suggest PFI-5 cleanly targets SMYD2...
by SERP+
(on 22 Mar 2023 )
Cellular Use Rating
No stability assay, protein binding or DMPK data are shown. Data regarding toxicity, hidden in the supplement, indicate a small experimental window.
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