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PF3644022 | PF3644022 : ATP competitive inhibitor of MAPKAPK2
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (3 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
MAPKAPK2
  • Ki:3 nM
  • Kd:5.9 nM
  • IC50:5.2 nM
  • IC50:200 nM
  • IC50:159 nM
ATP competitive
200-900 nM

Selectivity

In Vitro Selectivity Assessment

Potency: IC50 - MK3 53 nM, PRAK 53 nM, MNK2 148 nM, AMPK 117 nM, BrSK1 187 nM, BrSK2 90 nM, CAMK2 70 nM, DRAK1 71 nM, PIM1 88 nM, ASK1 60 nM, MER 76 nM

Potency Assay Off-Target:
At 1 uM, PF3644022 inhibited 16 kinases > 50% out of a panel of 200.
In Cell Selectivity Assessment
Selectivity Assessment Description:
PF36644022 did not interfere with the phosphorylation of substrates for p38alpha or JNK.

Potency
Cellular
In Vitro

MAPKAPK2

Mode of Action: ATP competitive

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1124/jpet.110.166173

In Vivo Validations

Rat
Dose: 3 mg/kg
Route of delivery: Oral
Plasma half life: 9.1 hr
Systemic clearance: 0.62 L/h/kg

DOI Reference: 10.1124/jpet.110.166173

Chemical Information

Molecular Formula C21H18N4OS
SMILEs Cc1ccc(-c2ccc3c(ccc4sc5c(c43)NC[C@@H](C)NC5=O)n2)cn1
InChI InChI=1S/C21H18N4OS/c1-11-3-4-13(10-22-11)15-6-5-14-16(25-15)7-8-17-18(14)19-20(27-17)21(26)24-12(2)9-23-19/h3-8,10,12,23H,9H2,1-2H3,(H,24,26)/t12-/m1/s1
Molecular weight 374.12 Da
AlogP 4.363720000000003
HBond acceptors 5
HBond donors 2
Atoms 45

References

Publications

Cross References

Vendors

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Expert Reviews

by SERP
(on 16 May 2016 )
Cellular Use Rating
In Model Organisms
( The reviewer did not leave any comments )
by SERP
(on 15 Jun 2016 )
Cellular Use Rating
In Model Organisms
...
by SERP
(on 9 Sept 2016 )
Cellular Use Rating
In Model Organisms
This compound was originally described by Pfizer in a publication in 2010 (J Pharmacol Exp Ther. 2010 Jun; 333(3): 797-807). It has high potency towards MK2 (Ki = 3 nM) as well as PRAK (IC50 = 5 nM),...
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