Chemical Probes logo
PF-477736 | PF-477736 : ATP-competitive inhbitor of CHEK1
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (3 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
Download

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
CHEK1
  • Ki:0.49 nM
  • EC50:45 nM
ATP competitive
50 nM - 1 uM

Selectivity

In Vitro Selectivity Assessment

Potency: IC50 - AURA 23 nM, FGFR3 23 nM, FLT3 25 nM, CSF1R 10 nM, RET 39 nM, YES 14 nM, VEGFR2 8 nM (Ki), CHEK2 47 nM (Ki)

Potency Assay Off-Target:
>100 kinases were screened, and PF-477136 was >100-fold selective for all other than those indicated ...

Potency
Cellular
In Vitro

CHEK1

Mode of Action: ATP competitive

Structure-Activity-Relationship data available? No

In Vivo Validations

nu/nu mouse (Sprague-Dawley rat)
Dose: 4, 20, and 40 mg/kg (4 mg/kg)
Route of delivery: Intraperitoneal, IV in rats
Plasma half life: 2.9 h (rats)
Systemic clearance: 11.8 mL/min/kg (rats)

Reference: --

Orthogonal Probes def

CCT244747

Chemical Information

Molecular Formula C22H25N7O2
SMILEs Cn1cc(-c2[nH]c3cc(NC(=O)[C@H](N)C4CCCCC4)cc4c(=O)[nH]ncc2c34)cn1
InChI InChI=1S/C22H25N7O2/c1-29-11-13(9-25-29)20-16-10-24-28-21(30)15-7-14(8-17(27-20)18(15)16)26-22(31)19(23)12-5-3-2-4-6-12/h7-12,19,27H,2-6,23H2,1H3,(H,26,31)(H,28,30)/t19-/m1/s1
Molecular weight 419.21 Da
AlogP 0.0
HBond acceptors 9
HBond donors 5
Atoms 56

References

Publications

    Cross References

    canSARChEMBLBindingDBPDB

    Vendors

    Note: This is not an exhaustive list and does not indicate endorsement by the portal.

    Expert Reviews

    by SERP
    (on 16 May 2016 )
    Cellular Use Rating
    In Model Organisms
    ( The reviewer did not leave any comments )
    by SERP
    (on 9 Jun 2016 )
    Cellular Use Rating
    In Model Organisms
    The compound abrogates cell cycle arrest induced by DNA damage and enhances cytotoxicity of clinically important chemotherapeutic agents, including gemcitabine and carboplatin. It also inhibits VEGFR2,...
    by SERP
    (on 22 Jun 2016 )
    Cellular Use Rating
    In Model Organisms
    This probe is a potent inhibitor of CHK1 in biochemical and cellular assays.
    Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria