PAMAPIMOD | PAMAPIMOD : Inhibitor of MAPK14A
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (2 reviews)
Control Compounds
Vendors

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
MAPK14
  • Kd:1.3 nM
  • IC50:14 ± 2 nM
  • IC50:25 ± 1 nM
  • IC50:100 ± 3 nM
Inhibitor
1 µM necessary for cellular activity
up to 1 uM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
Ligand displacement at 10 μM test compound was assessed using the Ambit Biosciences kinase screen of ...

Potency
Cellular
In Vitro

MAPK14

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/jm101423y

In Vivo Validations

Rat
Dose: 5 mg/Kg IV, PO
Route of delivery: Intravenous, Oral
Plasma half life: 4.04 h PO
Systemic clearance: 1.26 (L/h)/kg IV
Cmax: 1.34 ug/mL PO
Tmax: 0.5 h PO
Area Under the Curve:: 3.13 (24 h)μg·h/mL PO
Bioavailability: 80.1% PO
Volume of Distribution at Steady-State: 3.56 L/Kg IV

DOI Reference: 10.1021/jm101423y

Monkey (Cynomolgus)
Dose: 5 mg/Kg IV, PO
Route of delivery: Intravenous, Oral
Plasma half life: 13.8 h PO
Systemic clearance: 0.65 (L/h)/Kg IV
Cmax: 1.86 ug/mL PO
Tmax: 1.50 h PO
Area Under the Curve:: 9.41 (72h) μg·h/mL PO
Bioavailability: >100% PO
Volume of Distribution at Steady-State: 20.6 L/Kg IV

DOI Reference: 10.1021/jm101423y

Dog
Dose: 5 mg/Kg
Route of delivery: Intravenous, Oral
Plasma half life: 9.18 h PO
Systemic clearance: 0.75 (L/h)/kg IV
Cmax: 1.38 ug/mL PO
Tmax: 0.67 h PO
Area Under the Curve:: 5.22 μg·h/mL PO
Bioavailability: 78.2% PO
Volume of Distribution at Steady-State: 12.7 L/Kg IV

DOI Reference: 10.1021/jm101423y

Orthogonal Probes def

SR-318

Chemical Information

Molecular Formula C19H20F2N4O4
SMILEs Cn1c(=O)c(Oc2ccc(F)cc2F)cc2cnc(NC(CCO)CCO)nc21
InChI InChI=1S/C19H20F2N4O4/c1-25-17-11(10-22-19(24-17)23-13(4-6-26)5-7-27)8-16(18(25)28)29-15-3-2-12(20)9-14(15)21/h2-3,8-10,13,26-27H,4-7H2,1H3,(H,22,23,24)
Molecular weight 406.15 Da
AlogP 1.9442999999999997
HBond acceptors 8
HBond donors 3
Atoms 49

References

Publications

Cross References

Expert Reviews


(on 5 May 2025 )
Cellular Use Rating
In Model Organisms
This is a clinical candidate p38 inhibitor that has been demonstrated to be effective in an in vivo model of acute inflammation, such as rheumatoid arthritis. However, the results need to be carefully...
(on 5 May 2025 )
Cellular Use Rating
In Model Organisms
Pamapimod (compound 2b) is a highly potent, isoform-selective small-molecule inhibitor of p38α (MAPK14), a serine/threonine kinase central to inflammatory signaling pathways. Originally developed for the...
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