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PAD-PF2 | PAD-PF2 : allosteric inhibitor of PADI1, PADI2, PADI3 and PADI4
RATINGS:
Cellular Use: (1 reviews)

In Model Organisms: (0 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
PADI1
  • IC50:109 nM
    PADI2
    • IC50:28.5 nM
    • IC50:2.04 µM
      PADI3
      • IC50:106 nM
        PADI4
        • IC50:42.7 nM
        • IC50:24.0 nM
        • IC50:1.95 µM
        • IC50:1000 nM
        allosteric
        up to 10 uM

        Selectivity

        In Vitro Selectivity Assessment
        Potency Assay Off-Target:
        The target specificity of PAD-PF2 was evaluated by profiling the compound against a diverse panel of ...

        Potency
        Cellular
        In Vitro

        PADI1

        Mode of Action: allosteric

        Structure-Activity-Relationship data available? Yes

        DOI Reference: 10.1038/s41467-025-59919-4

        PADI2

        Mode of Action: allosteric

        Structure-Activity-Relationship data available? Yes

        DOI Reference: 10.1038/s41467-025-59919-4

        PADI3

        Mode of Action: allosteric

        Structure-Activity-Relationship data available? Yes

        DOI Reference: 10.1038/s41467-025-59919-4

        PADI4

        Mode of Action: allosteric

        Structure-Activity-Relationship data available? Yes

        DOI Reference: 10.1038/s41467-025-59919-4

        Negative Control Compounds

        canSAR7437551
        Notes: The enantiomer of PAD-PF2 (ent-PAD-PF2) shows an IC50 = 26.0 µM in FQ assay.

        Chemical Information

        Molecular Formula C25H27Cl2N5O
        SMILEs N[C@H](C(=O)N1CCCC1)C1CCN(c2nccc3c(-c4ncc(Cl)cc4Cl)cccc23)CC1
        InChI InChI=1S/C25H27Cl2N5O/c26-17-14-21(27)23(30-15-17)19-4-3-5-20-18(19)6-9-29-24(20)31-12-7-16(8-13-31)22(28)25(33)32-10-1-2-11-32/h3-6,9,14-16,22H,1-2,7-8,10-13,28H2/t22-/m0/s1
        Molecular weight 483.16 Da
        AlogP 4.769700000000004
        HBond acceptors 6
        HBond donors 2
        Atoms 60

        References

        Publications

        Cross References

        Expert Reviews

        by SERP
        (on 27 May 2025 )
        Cellular Use Rating
        Overall, this probe seems to be clearly investigated and the data in the paper (DOI: 10.1038/s41467-025-59919-4) is in-depth. This probe is definitely useful as a PAD probe, but may be useful in combination...
        Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria