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MSC778 | MSC778 : Inhibitor of FEN1
RATINGS:
Cellular Use: (1 reviews)

In Model Organisms: (1 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
FEN1
  • IC50:3.0 nM
  • Kd:2.9 nM
  • EC50:31 nM
  • EC50:210 nM
Inhibitor
up to 1 uM

Selectivity

In Vitro Selectivity Assessment
Selectivity Assessment Description:
Selectivity against other nucleases: EXO1 145-fold GEN1 516-fold XPG 65-folda
In Vitro Selectivity Assessment
Selectivity Assessment Description:
MSC778 displayed excellent selectivity against all members of an in-house panel of the broader nucle ...

Potency
Cellular
In Vitro

FEN1

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acs.jmedchem.5c01526

In Vivo Validations

Mouse
Dose: 0.2 mg/Kg IV, 10, 30 mg/Kg PO
Route of delivery: Intravenous, Oral
Plasma half life: 4.1 h
Systemic clearance: 0.39 mL/min/Kg
Bioavailability: 16%
Volume of Distribution at Steady-State: 0.1 L/Kg

DOI Reference: 10.1021/acs.jmedchem.5c01526

Rat
Dose: 0.2 mg/Kg IV, 10 mg/Kg PO
Route of delivery: Intravenous, Oral
Plasma half life: 2.9 h
Systemic clearance: 1.5 mL/min/Kg
Bioavailability: 15%
Volume of Distribution at Steady-State: 0.2 L/Kg

DOI Reference: 10.1021/acs.jmedchem.5c01526

Dog
Dose: 0.2 mg/Kg IV, 16 mg/Kg PO
Route of delivery: Intravenous, Oral
Plasma half life: 3.6 h
Systemic clearance: 1.3 mL/min/Kg
Bioavailability: 21%
Volume of Distribution at Steady-State: 0.4 L/Kg

DOI Reference: 10.1021/acs.jmedchem.5c01526

Negative Control Compounds

canSAR7437560
Notes: (S,R)-31 had a weather Kd (23.3 nM - 10 fold), NanoBRET EC50 (506 nM) and phenotypic assay (BRCA2 KO viability assay EC50 4850 nM)

Chemical Information

Molecular Formula C22H20ClN3O5
SMILEs C[C@H](Oc1nn2c(c(O)c1=O)C(=O)N1CCOC[C@@H]1C2)c1ccc2ccc(Cl)cc2c1
InChI InChI=1S/C22H20ClN3O5/c1-12(14-3-2-13-4-5-16(23)9-15(13)8-14)31-21-20(28)19(27)18-22(29)25-6-7-30-11-17(25)10-26(18)24-21/h2-5,8-9,12,17,27H,6-7,10-11H2,1H3/t12-,17-/m0/s1
Molecular weight 441.11 Da
AlogP 2.7501000000000007
HBond acceptors 8
HBond donors 1
Atoms 51

References

Publications

Cross References

Expert Reviews

by SERP
(on 18 Dec 2025 )
Cellular Use Rating
In Model Organisms
MSC778 is a potent FEN1 nuclease inhibitor, as validated by a range of biochemical and biophysical assays (endonuclease activity and SPR). Extensive SAR supports this conclusion, as well as the availability...
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