MDI-114215 | MDI-114215 : Inhibitor of LIMK1, LIMK2
RATINGS:
Cellular Use: (1 reviews)

In Model Organisms: (1 reviews)
Control Compounds
Vendors

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
LIMK1
  • Kd:33 nM
  • IC50:13 nM
  • IC50:46 nM
  • IC50:50 nM
LIMK2
  • Kd:40 nM
  • IC50:11 nM
  • IC50:50 nM
Inhibitor
up to 300 nM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
Wider kinome profiling was performed on MDI-114215 using the Eurofins/DiscoverX scanMAX panel of 468 ...
In Vitro Selectivity Assessment
Potency Assay Off-Target:
Off-target pharmacological activity in a panel of receptors, ion channels, transporters and enzymes ...

Potency
Cellular
In Vitro

LIMK1

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acs.jmedchem.4c02694

LIMK2

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acs.jmedchem.4c02694

In Vivo Validations

Mouse
Dose: 0.2 mg/Kg
Route of delivery: Intravenous
Plasma half life: 0.6 h
Systemic clearance: 33.1 mL/min/kg
Area Under the Curve:: 101 h ng/mL
Volume of Distribution at Steady-State: 1.3 L/Kg

DOI Reference: 10.1021/acs.jmedchem.4c02694

Dose: 30 mg/Kg
Route of delivery: Intraperitoneal
Cmax: 1385 ng/mL
Tmax: 0.4 h
Bioavailability: 52%

DOI Reference: 10.1021/acs.jmedchem.4c02694

Orthogonal Probes def

Chemical Information

Molecular Formula C26H29N3O4S
SMILEs O=C(c1ccc(S(=O)(=O)Nc2ccccc2)cc1)N(Cc1ccc(NCCO)cc1)CC1CC1
InChI InChI=1S/C26H29N3O4S/c30-17-16-27-23-12-8-21(9-13-23)19-29(18-20-6-7-20)26(31)22-10-14-25(15-11-22)34(32,33)28-24-4-2-1-3-5-24/h1-5,8-15,20,27-28,30H,6-7,16-19H2
Molecular weight 479.19 Da
AlogP 3.9440000000000026
HBond acceptors 7
HBond donors 3
Atoms 63

References

Cross References

Expert Reviews


(on 25 Feb 2025 )
Cellular Use Rating
In Model Organisms
Despite several reported tool compounds for LIMK1/2 many of which suffer from low overall kinase selectivity. The dual LIMK1/2 inhibitor MDI-114215 is a well-characterized chemical probe. It shows good...
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