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MASTL-IN-1 | MASTL-IN-1 : Inhibitor of MASTL
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (3 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
MASTL
  • Ki:0.03 nM
  • IC50:1.1 nM
  • IC50:3.0 nM
Inhibitor
up to 100 nM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
MASTL-IN-15 was profiled against a panel of 394 kinases for percent inhibition at 100 nM (>3,000x ov ...

Potency
Cellular
In Vitro

MASTL

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acs.jmedchem.4c01659

In Vivo Validations

Mouse
Dose: 0.5 mg/Kg IV, 30 mg/Kg PO
Route of delivery: Intravenous, Oral
Plasma half life: 1.2 h
Systemic clearance: 109 mL/min/Kg
Cmax: 725 ng/mL PO
Tmax: 2.0 h PO
Area Under the Curve:: 76.2 IV, 4400 PO ng*h/mL
Bioavailability: 96% PO
Volume of Distribution at Steady-State: 4.9 L/Kg

DOI Reference: 10.1021/acs.jmedchem.4c01659

Chemical Information

Molecular Formula C21H25N9
SMILEs CCC(N)(CC)c1ccnc(Nc2cc3cc(-c4cnnn4C)nc(N)c3cn2)c1
InChI InChI=1S/C21H25N9/c1-4-21(23,5-2)14-6-7-24-19(10-14)28-18-9-13-8-16(17-12-26-29-30(17)3)27-20(22)15(13)11-25-18/h6-12H,4-5,23H2,1-3H3,(H2,22,27)(H,24,25,28)
Molecular weight 403.22 Da
AlogP 3.1201000000000008
HBond acceptors 9
HBond donors 5
Atoms 55

Expert Reviews

by SERP
(on 16 Dec 2024)
Cellular Use Rating
In Model Organisms
Currently, this probe has been used in a single publication. While the data provided in this publication is generally promising, independent validation is needed in order to recommend this probe for future...
by SERP
(on 19 Dec 2024)
Cellular Use Rating
In Model Organisms
Highly potent inhibitor of MASTL with excellent (>50 fold) selectivity in a kinase screen. Target engagement in cells confirmed via S67 phosphorylation. No cytoxocity data provided.
by SERP
(on 27 Jan 2025)
Cellular Use Rating
In Model Organisms
MASTL-IN-1 is highly potent (Ki <0.03 nM). The recommended cellular dose (10 nM; and <100 nM) should be followed to observe the compound's selectivity. In vivo study showed the IC90 could be covered sufficiently...
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