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MASTL-IN-1 | MASTL-IN-1 : Inhibitor of MASTL
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (3 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
MASTL
  • Ki:0.03 nM
  • IC50:1.1 nM
  • IC50:3.0 nM
Inhibitor
up to 100 nM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
MASTL-IN-15 was profiled against a panel of 394 kinases for percent inhibition at 100 nM (>3,000x ov ...

Potency
Cellular
In Vitro

MASTL

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acs.jmedchem.4c01659

In Vivo Validations

Mouse
Dose: 0.5 mg/Kg IV, 30 mg/Kg PO
Route of delivery: Intravenous, Oral
Plasma half life: 1.2 h
Systemic clearance: 109 mL/min/Kg
Cmax: 725 ng/mL PO
Tmax: 2.0 h PO
Area Under the Curve:: 76.2 IV, 4400 PO ng*h/mL
Bioavailability: 96% PO
Volume of Distribution at Steady-State: 4.9 L/Kg

DOI Reference: 10.1021/acs.jmedchem.4c01659

Chemical Information

Molecular Formula C21H25N9
SMILEs CCC(N)(CC)c1ccnc(Nc2cc3cc(-c4cnnn4C)nc(N)c3cn2)c1
InChI InChI=1S/C21H25N9/c1-4-21(23,5-2)14-6-7-24-19(10-14)28-18-9-13-8-16(17-12-26-29-30(17)3)27-20(22)15(13)11-25-18/h6-12H,4-5,23H2,1-3H3,(H2,22,27)(H,24,25,28)
Molecular weight 403.22 Da
AlogP 3.1201000000000008
HBond acceptors 9
HBond donors 5
Atoms 55

Expert Reviews

by SERP
(on 16 Dec 2024 )
Cellular Use Rating
In Model Organisms
Currently, this probe has been used in a single publication. While the data provided in this publication is generally promising, independent validation is needed in order to recommend this probe for future...
by SERP
(on 19 Dec 2024 )
Cellular Use Rating
In Model Organisms
Highly potent inhibitor of MASTL with excellent (>50 fold) selectivity in a kinase screen. Target engagement in cells confirmed via S67 phosphorylation. No cytoxocity data provided.
by SERP
(on 27 Jan 2025 )
Cellular Use Rating
In Model Organisms
MASTL-IN-1 is highly potent (Ki <0.03 nM). The recommended cellular dose (10 nM; and <100 nM) should be followed to observe the compound's selectivity. In vivo study showed the IC90 could be covered sufficiently...
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