LC-04-45

LC-04-45 : Molecular Glue of NEK7

Structure

SERP Rating

In Cells

(2 ratings)

In Model Organisms

(0 ratings)

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Probe LC-04-45 is in the process of SERP review.

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Information

NEK7

Molecular Glue

up to 1 uM

In Vitro Validations

No in Vitro Validations

In Cell Validations

In Vivo Data

No in Vivo Validations

Off-Target Selectivity Assesments

Potency assay, off target (cells): Proteome-wide selectivity was evaluated in MOLT-4 cells treated with 1 μM of LC-04-045 or DMSO for 6 h. Only NEK7 was significantly affected by LC-04-045 tratement.

Potency assay, off target (cells): Selectivity against NEK1, NEK2, NEK4, NEK7, NEK10 and NEK11 was assessed via immunoblot in MOLLT4 cells for 6h and showed high selectivity for NEK7.

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SERP ratings and comments

SERP Ratings

In Cell Rating

(last updated: 21 Jan 2026 )

SERP Ratings

In Cell Rating

SERP Comments:

LC-04-45 is a potent CRBN-dependent NEK7 glue degrader, with established selectivity in MOLT-4 cells (human T lymphoblast cell line) at short time point (6h). LC-04-45-induced degradation of NEK7 has been validated in a limited number of cell types: MOLT-4, human PBMC, and human monocyte–derived macrophages, with Dmax > 85% and DC50 < 100 nM across all cell types tested. Proof of mechanism was obtained using a cellular split nanoluciferase assay: LC-04-45 could show recruitment of SmBiT-CRBN to LgBit-NEK7 in dose dependent manner in HEK293T cells. However, no biophysical assay or structural biology study using purified protein is reported. Selectivity data obtained in MOLT-4 cells treated with LC-04-45 for 6h appear clean both regarding whole proteomics data and targeted WB on NEK family. Potential caveats include the lack of data at longer timepoints and in different cell types. Overall, LC-04-45 is a minimally validated chemical probe for NEK7 degradation, that I would recommend to use mostly in complement to more validated NEK7 degrader NK7-902 (https://www.biorxiv.org/content/biorxiv/early/2024/11/08/2024.11.06.622079.full.pdf).

(last updated: 24 Jan 2026 )