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JRD-SIK1/2i-4 | JRD-SIK1/2i-4 : Inhibitor of SIK1, SIK2
RATINGS:
Cellular Use: (1 reviews)

In Model Organisms: (1 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
SIK1
  • IC50:143 nM
  • Ki:3.1 nM
    SIK2
    • IC50:76 nM
    • Ki:1.9 nM
    • EC50:700 nM
    • IC50:110 nM
    • IC50:250 nM
    • EC50:2910 nM
    Inhibitor
    5 -10 µM
    up to 5 uM

    Selectivity

    In Vitro Selectivity Assessment
    Potency Assay Off-Target:
    Selectivity within target family: SIK isoform selectivity was assessed in SIK1, SIK2, and SIK3 ADP-G ...

    Potency
    Cellular
    In Vitro

    SIK1

    Mode of Action: Inhibitor

    Structure-Activity-Relationship data available? No

    DOI Reference: 10.1073/pnas.2307086120

    SIK2

    Mode of Action: Inhibitor

    Structure-Activity-Relationship data available? No

    DOI Reference: 10.1073/pnas.2307086120

    In Vivo Validations

    Rat
    Dose: 1 mg/Kg IV, 5 mg/Kg PO
    Route of delivery: Intravenous, Oral
    Systemic clearance: 59 mL/min/Kg (IV)
    Bioavailability: 60% (PO)
    Volume of Distribution at Steady-State: 4.0 L/Kg (IV)

    DOI Reference: 10.1073/pnas.2307086120

    Mouse
    Dose: 20 and 80 mg/Kg
    Route of delivery: Oral

    DOI Reference: 10.1073/pnas.2307086120

    Chemical Information

    Molecular Formula C21H20N6O2
    SMILEs CN1CC(Oc2cnc(C#N)cc2-c2ccn3nc(NC(=O)C4CC4)cc3c2)C1
    InChI InChI=1S/C21H20N6O2/c1-26-11-17(12-26)29-19-10-23-15(9-22)7-18(19)14-4-5-27-16(6-14)8-20(25-27)24-21(28)13-2-3-13/h4-8,10,13,17H,2-3,11-12H2,1H3,(H,24,25,28)
    Molecular weight 388.16 Da
    AlogP 0.0
    HBond acceptors 8
    HBond donors 1
    Atoms 49

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    Expert Reviews

    by SERP
    (on 26 Mar 2024 )
    Cellular Use Rating
    In Model Organisms
    Direct mechanistic SIK1/SIK2 inhibition in cells was analysed by phosphorylation of CRTC3 (Supplemental Figure S2 in DOI 10.1073/pnas.2307086120. There were insufficient data points to calculate an IC50...
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