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JNJ-47117096 | JNJ-47117096 : Inhibitor of MELK
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (0 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

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Targets Biochemical/Biophysical Potency Cellular Potency
MELK
  • IC50:37 nM
  • IC50:200 nM
  • IC50:520 nM
  • EC50:>10 µM
Inhibitor
higher concentrations of up to 5 µM or 10 µM
1 µM

Selectivity

In Vitro Selectivity Assessment
FLT3

Gene ID: P36888

Organism: Homo sapiens

Family: Tyr protein kinase family

Potency: IC50 - 18 nM

Potency Assay Off-Target:
Radioactive filter binding assay (10 µM ATP)
Selectivity Assessment Description:
Screened at 1 µM against 235 kinases, KinaseProfile service (Millipore). Closest targets as % of con ...
In Cell Selectivity Assessment
Potency Assay Off-Target:
inhibition of proliferation with IC50 1.5 μM in the absence of IL-3 in Ba/F3-Flt3 cells but no inh ...

Potency
Cellular
In Vitro

MELK

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/ml5001245

Orthogonal Probes def

NVS-MELK8a
ChEMBL ID: CHEMBL3824068

Chemical Information

Molecular Formula C21H22N4O2
SMILEs COc1cc(-c2cn[nH]c2)ccc1C(=O)Nc1ccc2c(c1)CCNCC2
InChI InChI=1S/C21H22N4O2/c1-27-20-11-15(17-12-23-24-13-17)3-5-19(20)21(26)25-18-4-2-14-6-8-22-9-7-16(14)10-18/h2-5,10-13,22H,6-9H2,1H3,(H,23,24)(H,25,26)
Molecular weight 362.17 Da
AlogP 3.025800000000001
HBond acceptors 6
HBond donors 3
Atoms 49

Vendors

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Expert Reviews

by SERP+
(on 23 Sept 2024)
Cellular Use Rating
(The reviewer did not leave any public comments)
by SERP
(on 7 Oct 2024)
Cellular Use Rating
This compound has utility as a probe, however several caveats should be considered when utilizing. 1) This compound shows strong kinase activity for Flt3 (18 nM), which is comparable to the measured IC50...
by SERP
(on 2 Jan 2025)
Cellular Use Rating
The probe JNJ-47117096 has potential to be useful as an orthogonal reagent for cellular validation of MELK in combination with either genetic knockdown or other structurally distinct MELK small molecule...
Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria