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JNJ-42396302 | JNJ-42396302 : Inhibitor of PDE10A
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (2 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
PDE10A
  • Ki:13 nM
  • IC50:40 nM
  • IC50:51 nM
  • Activity:2.3 fold increase @ 1uM
Inhibitor
up to 10 uM

Selectivity

In Vitro Selectivity Assessment
Selectivity Assessment Description:
Selectivity within target family: PDE selectivity > 250 fold: closest hit is PDE1B with Ki = 3.4 µM ...

Potency
Cellular
In Vitro

PDE10A

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? No

DOI Reference: 10.6019/CHEMBL4800727

In Vivo Validations

Rat
Dose: 10 mg/Kg
Route of delivery: Oral
Target engagement assay: Dose-and time-dependent occupancy of rat brain PDE10 shown: Maximum occupancy was observed 1 hour after p.o. dosing (ED50: 1.4 mg/kg). The time-course experiment showed a gradual decrease of PDE10 occupancy over time, with 50% occupancy up to 8 h after dosing (10 mg/kg, p.o.).

DOI Reference: 10.6019/CHEMBL4800727

Negative Control Compounds

JNJ-40573663
Notes: JNJ-40573663: human PDE10A IC50 > 10 µM (in vitro assay)
InChI: InChI=1S/C21H25N5O/c1-2-4-17(5-3-1)18-6-9-24(10-7-18)19-16-23-21-20(22-8-11-26(19)21)25-12-14-27-15-13-25/h1-5,8,11,16,18H,6-7,9-10,12-15H2

Orthogonal Probes def

THPP-1

Chemical Information

Molecular Formula C19H23N5O2
SMILEs COCCc1ccc(-c2c(C)nc3c(N4CCOCC4)ccnn23)cn1
InChI InChI=1S/C19H23N5O2/c1-14-18(15-3-4-16(20-13-15)6-10-25-2)24-19(22-14)17(5-7-21-24)23-8-11-26-12-9-23/h3-5,7,13H,6,8-12H2,1-2H3
Molecular weight 353.19 Da
AlogP 2.1252199999999997
HBond acceptors 7
HBond donors --
Atoms 49

Vendors

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Expert Reviews

by SERP
(on 1 Jul 2022 )
Cellular Use Rating
In Model Organisms
Based on the information provided in the pdf file the compound looks potent, selective, and cell-permeable. I would recommend to use JNJ-42396302 as a chemical probe both in vitro and in cell-based assays....
by SERP
(on 4 Jul 2022 )
Cellular Use Rating
In Model Organisms
Limited cell assay data is made available, with the increase in observed effects between 2.3 and 10 µM suggesting a cellular potency >5 µM (significant uncertainty in this estimate). In addition, cell...
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