JNJ-3738 | JNJ-3738 : Covalent Inhibitor of CDK7
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (2 reviews)
Vendors

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
CDK7
  • IC50:58 nM
  • IC50:4 nM
Covalent Inhibitor
up to 1 uM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
Selectivity within target family: Good CDK familiy selectivity: Closest family member is CDK9 (45 % ...

Potency
Cellular
In Vitro

CDK7

Mode of Action: Covalent Inhibitor

Structure-Activity-Relationship data available? No

Other Reference: Peer reviewed at SGC

In Vivo Validations

Mouse
Dose: 0.5 mg/Kg
Route of delivery: Intravenous
Plasma half life: 1.49 h
Systemic clearance: 85 mL/min/kg
Volume of Distribution at Steady-State: 4.36 L/Kg

Other Reference: Peer reviewed at SGC

Dose: 5 mg/Kg
Route of delivery: Oral
Cmax: 0.11 uM
Tmax: 1.67 h
Area Under the Curve:: 0.43 mM*h
Bioavailability: 25%

Other Reference: Peer reviewed at SGC

Rat
Dose: 1 mg/Kg
Route of delivery: Intravenous
Systemic clearance: 66 mL/min/Kg
Volume of Distribution at Steady-State: 2.96 L/Kg

Other Reference: Peer reviewed at SGC

Dose: 5 mg/Kg
Route of delivery: Oral
Cmax: 0.11 uM
Tmax: 1.00 h
Area Under the Curve:: 0.67 mM*h
Bioavailability: 29%

Other Reference: Peer reviewed at SGC

Dog
Dose: 1 mg/Kg
Route of delivery: Intravenous
Plasma half life: 1.81 h
Systemic clearance: 19 mL/min/kg
Volume of Distribution at Steady-State: 2.82 L/Kg

Other Reference: Peer reviewed at SGC

Dose: 5 mg/Kg
Route of delivery: Oral
Cmax: 0.33 uM
Tmax: 1.67 h
Area Under the Curve:: 2.02 mM*h
Bioavailability: 27%

Other Reference: Peer reviewed at SGC

Negative Control Compounds

JNJ-6240
Notes: JNJ-6240: IC50 ~ 200 nM (KMATP app ~30 µM; fluorescent end-point assay); PD: Detection of p-RNA pol II Ser 5 in A549 cells after 3 hrs incubation with probe: IC50 > 10 µM; C312S mutant: IC50 > 10 µM
SMILES: CC(N1CC(C1)N1CCC(CC1)c1cc(C)c2c(c1)Nc1c(c(ncn1)N1CCS(CC1)(=O)=O)[C@@H](C)O2)=O

Chemical Information

Molecular Formula C29H36N6O4S
SMILEs CC#CC(=O)N1CC(N2CCC(c3cc(C)c4c(c3)Nc3ncnc(N5CCS(=O)(=O)CC5)c3[C@@H](C)O4)CC2)C1
InChI InChI=1S/C29H36N6O4S/c1-4-5-25(36)35-16-23(17-35)33-8-6-21(7-9-33)22-14-19(2)27-24(15-22)32-28-26(20(3)39-27)29(31-18-30-28)34-10-12-40(37,38)13-11-34/h14-15,18,20-21,23H,6-13,16-17H2,1-3H3,(H,30,31,32)/t20-/m1/s1
Molecular weight 564.25 Da
AlogP 0.0
HBond acceptors 10
HBond donors 1
Atoms 76

Expert Reviews


(on 18 Dec 2024 )
Cellular Use Rating
In Model Organisms
( The reviewer did not leave any comments )
(on 3 Feb 2025 )
Cellular Use Rating
In Model Organisms
JNJ-3738 is a potent, irreversible covalent inhibitor specifically targeting CDK7. It exhibits remarkable selectivity for CDK7 over other CDKs, due to its binding to the cysteine 312 residue unique to...
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