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JH-X-119-01 | JH-X-119-01 : Covalent inhibitor of IRAK1 and YSK4
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (1 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
IRAK1
  • IC50:9 nM
Covalent
1-10 µM
up to 1 uM

Selectivity

In Vitro Selectivity Assessment
MAP3K19

Gene ID: Q56UN5

Organism: Homo sapiens

Family: STE Ser/Thr protein kinase family

Potency: IC50 - 57 nM

Potency Assay Off-Target:
JH-X-119-01 showed exceptional kinome selectivity, as measured by a KINOMEScan selectivity score of ...

Potency
Cellular
In Vitro

IRAK1

Mode of Action: Covalent

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acsmedchemlett.0c00378

In Vivo Validations

Mouse
Dose: 5 mg/kg
Route of delivery: Intravenous
Plasma half life: 2.24 h
Systemic clearance: 18.8 ml/min/Kg
Cmax: Cmax = 9.95 µM
Volume of Distribution at Steady-State: Vss=0.46 L/Kg

DOI Reference: 10.1021/acsmedchemlett.0c00378

Chemical Information

Molecular Formula C25H20N6O3
SMILEs C=CC(=O)Nc1cccc(C(=O)Nc2ccc(NC(=O)c3cccc(-c4ccn[nH]4)n3)cc2)c1
InChI InChI=1S/C25H20N6O3/c1-2-23(32)27-19-6-3-5-16(15-19)24(33)28-17-9-11-18(12-10-17)29-25(34)22-8-4-7-20(30-22)21-13-14-26-31-21/h2-15H,1H2,(H,26,31)(H,27,32)(H,28,33)(H,29,34)
Molecular weight 452.16 Da
AlogP 0.0
HBond acceptors 9
HBond donors 4
Atoms 54

Vendors

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Expert Reviews

by SERP
(on 16 Feb 2023)
Cellular Use Rating
In Model Organisms
The IRAK1 inhibitory selectivity against IRAK4 is extremely high, so this probe should be useful for assessing sole IRAK1 activity.
by SERP
(on 22 Feb 2023)
Cellular Use Rating
JH-X-119-01 is a well-characterized, selective modulator of IRAK1 relative to IRAK4. Cell activity depends on the line used. As such it is recommended that researchers conduct a dose-reponse as well cytoxicity...
Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria