IMT1B | IMT1B : Allosteric inhibitor of POLRMT
RATINGS:
Cellular Use: (0 reviews)

In Model Organisms: (0 reviews)
Vendors

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
POLRMT
  • ΔTm:5 K
  • IC50:about 50 nM
  • IC50:748 nM
  • Kd:100 nM
  • Ratio:~40%
Allosteric
up to 10 uM

Selectivity

In Cell Selectivity Assessment
Potency Assay Off-Target:
IMT1B did not inhibit budding yeast mitochondrial RNA polymerase (RPO41), bacteriophage T7 RNA polym ...

Potency
Cellular
In Vitro

POLRMT

Mode of Action: Allosteric

Structure-Activity-Relationship data available? No

DOI Reference: 10.1038/s41586-020-03048-z

In Vivo Validations

Mouse
Dose: 1 mg/Kg
Route of delivery: Intravenous
Plasma half life: 1.88 h
Systemic clearance: 0.44 L/h/Kg
Area Under the Curve:: 2256 h*ng/mL
Volume of Distribution at Steady-State: 1.2 L/Kg

DOI Reference: 10.1038/s41586-020-03048-z

Dose: 10 mg/Kg
Route of delivery: Oral
Plasma half life: 2.72 h
Cmax: 5149 ng/mL
Tmax: 1.0 h
Area Under the Curve:: 22854 h*ng/mL
Bioavailability: 101%

DOI Reference: 10.1038/s41586-020-03048-z

Negative Control Compounds

SC-6238532
Notes: SC-6238532 (con IMT) is structurally related to IMTs—and it neither binds POLRMT nor affects mtDNA expression.
SMILES: CC1=C(OC(C(C(C)(C)C)=O)C)C=CC2=C1OC(C=C2C3=CC=CC=C3)=O

Chemical Information

Molecular Formula C24H21ClFNO6
SMILEs C[C@@H](Oc1ccc2c(-c3ccc(F)cc3Cl)cc(=O)oc2c1)C(=O)N1CCC[C@H](C(=O)O)C1
InChI InChI=1S/C24H21ClFNO6/c1-13(23(29)27-8-2-3-14(12-27)24(30)31)32-16-5-7-18-19(11-22(28)33-21(18)10-16)17-6-4-15(26)9-20(17)25/h4-7,9-11,13-14H,2-3,8,12H2,1H3,(H,30,31)/t13-,14+/m1/s1
Molecular weight 473.10 Da
AlogP 4.343000000000003
HBond acceptors 7
HBond donors 1
Atoms 54

References

Cross References

Expert Reviews


No SERP comments found for IMT1B

Probe IMT1B is in the process of SERP review.

Please continue to check back for new reviews and commentary.