Chemical Probes logo
I-CBP112 | I-CBP112 : Inhibitor of EP300, CREBBP
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (3 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
Download

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
EP300
  • Kd:167 nM
    CREBBP
    • Kd:151 nM
    • IC 50:170 nM
    • IC50:600 nM
    Inhibitor
    Up to 1 uM

    Selectivity

    In Vitro Selectivity Assessment

    Potency: KD - BRD4 (1) 5.6 uM (2) 20 uM

    Potency Assay Off-Target:
    ITC, and using biointerferometry, I-CBP112 did not bind to any additional of 40 Bromodomains tested. ...
    Selectivity Assessment Description:
    Minimal activity against 104 proteins (nuclear receptors, ion channels, kinases, proteases, and p ...

    Potency
    Cellular
    In Vitro

    EP300

    Mode of Action: Inhibitor

    Structure-Activity-Relationship data available? Yes

    DOI Reference: 10.1158/0008-5472.CAN-15-0236

    CREBBP

    Mode of Action: Inhibitor

    Structure-Activity-Relationship data available? Yes

    DOI Reference: 10.1158/0008-5472.CAN-15-0236

    In Vivo Validations

    Mouse
    Dose: 3 mg/mL
    Route of delivery: Intraperitoneal
    Plasma half life: 0.6 hr
    Organ of interest (O): bone marrow transplant with MLL-AF9 leukemia cells

    DOI Reference: 10.1158/0008-5472.CAN-15-0236

    Orthogonal Probes def

    SGC-CBP30
    GNE-781

    Chemical Information

    Molecular Formula C27H36N2O5
    SMILEs CCC(=O)N1CCOc2c(cc(-c3ccc(OC)c(OC)c3)cc2OC[C@H]2CCCN(C)C2)C1
    InChI InChI=1S/C27H36N2O5/c1-5-26(30)29-11-12-33-27-22(17-29)13-21(20-8-9-23(31-3)24(14-20)32-4)15-25(27)34-18-19-7-6-10-28(2)16-19/h8-9,13-15,19H,5-7,10-12,16-18H2,1-4H3/t19-/m0/s1
    Molecular weight 468.26 Da
    AlogP 4.2224
    HBond acceptors 7
    HBond donors --
    Atoms 70

    References

    Publications

    Cross References

    canSARChEMBLBindingDB

    Vendors

    Note: This is not an exhaustive list and does not indicate endorsement by the portal.

    Expert Reviews

    by SERP
    (on 20 Jan 2017 )
    Cellular Use Rating
    In Model Organisms
    ( The reviewer did not leave any comments )
    by SERP
    (on 31 Mar 2017 )
    Cellular Use Rating
    In Model Organisms
    I-CBP112 is a ligand of the highly conserved bromodomain of both CBP and p300. In vitro, the probe displays selective binding to CBP/p300 bromodomains with a Kd ~600 nM. Comparatively, the Kd for the...
    by SERP
    (on 6 Apr 2017 )
    Cellular Use Rating
    In Model Organisms
    Bromodomain probe with selectivity for CBP/p300 outside of the BET family. This probe is able to displace p300 and CBP from acetylated proteins at low micromolar concentrations. Use at concentrations...
    Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria