Chemical Probes logo
I-CBP112 | I-CBP112 : Inhibitor of EP300, CREBBP
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (3 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
Download

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
EP300
  • Kd:167 nM
    CREBBP
    • Kd:151 nM
    • IC 50:170 nM
    • IC50:600 nM
    Inhibitor
    Up to 1 uM

    Selectivity

    In Vitro Selectivity Assessment

    Potency: KD - BRD4 (1) 5.6 uM (2) 20 uM

    Potency Assay Off-Target:
    ITC, and using biointerferometry, I-CBP112 did not bind to any additional of 40 Bromodomains tested. ...
    Selectivity Assessment Description:
    Minimal activity against 104 proteins (nuclear receptors, ion channels, kinases, proteases, and p ...

    Potency
    Cellular
    In Vitro

    EP300

    Mode of Action: Inhibitor

    Structure-Activity-Relationship data available? Yes

    DOI Reference: 10.1158/0008-5472.CAN-15-0236

    CREBBP

    Mode of Action: Inhibitor

    Structure-Activity-Relationship data available? Yes

    DOI Reference: 10.1158/0008-5472.CAN-15-0236

    In Vivo Validations

    Mouse
    Dose: 3 mg/mL
    Route of delivery: Intraperitoneal
    Plasma half life: 0.6 hr
    Organ of interest (O): bone marrow transplant with MLL-AF9 leukemia cells

    DOI Reference: 10.1158/0008-5472.CAN-15-0236

    Orthogonal Probes def

    SGC-CBP30
    GNE-781

    Chemical Information

    Molecular Formula C27H36N2O5
    SMILEs CCC(=O)N1CCOc2c(cc(-c3ccc(OC)c(OC)c3)cc2OC[C@H]2CCCN(C)C2)C1
    InChI InChI=1S/C27H36N2O5/c1-5-26(30)29-11-12-33-27-22(17-29)13-21(20-8-9-23(31-3)24(14-20)32-4)15-25(27)34-18-19-7-6-10-28(2)16-19/h8-9,13-15,19H,5-7,10-12,16-18H2,1-4H3/t19-/m0/s1
    Molecular weight 468.26 Da
    AlogP 4.2224
    HBond acceptors 7
    HBond donors --
    Atoms 70

    References

    Publications

    Cross References

    canSARChEMBLBindingDB

    Vendors

    Note: This is not an exhaustive list and does not indicate endorsement by the portal.

    Expert Reviews

    by SERP
    (on 20 Jan 2017)
    Cellular Use Rating
    In Model Organisms
    (The reviewer did not leave any public comments)
    by SERP
    (on 31 Mar 2017)
    Cellular Use Rating
    In Model Organisms
    I-CBP112 is a ligand of the highly conserved bromodomain of both CBP and p300. In vitro, the probe displays selective binding to CBP/p300 bromodomains with a Kd ~600 nM. Comparatively, the Kd for the...
    by SERP
    (on 6 Apr 2017)
    Cellular Use Rating
    In Model Organisms
    Bromodomain probe with selectivity for CBP/p300 outside of the BET family. This probe is able to displace p300 and CBP from acetylated proteins at low micromolar concentrations. Use at concentrations...
    Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria