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GNF-5 | GNF-5 : Allosteric inhibitor of ABL1
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (3 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
ABL1
  • IC50 :0.22 nM
  • IC50:0.22 uM
Allosteric
500 nM - 5 uM

Selectivity

Potency
Cellular
In Vitro

ABL1

Mode of Action: Allosteric

Structure-Activity-Relationship data available? No

In Vivo Validations

BALB/c mice
Dose: 20 mg/kg
Route of delivery: Oral
Plasma half life: 2.30 h
Organ of interest (O): bone marrow
Target engagement assay: Indirect, inhibition of BCR-ABL signaling in bone marrow; substrate phosphorylation levels detected by flow cytometry.

Reference: --

Orthogonal Probes def

IMATINIB
BAFETINIB
ASCIMINIB

Chemical Information

Molecular Formula C20H17F3N4O3
SMILEs O=C(NCCO)c1cccc(-c2cc(Nc3ccc(OC(F)(F)F)cc3)ncn2)c1
InChI InChI=1S/C20H17F3N4O3/c21-20(22,23)30-16-6-4-15(5-7-16)27-18-11-17(25-12-26-18)13-2-1-3-14(10-13)19(29)24-8-9-28/h1-7,10-12,28H,8-9H2,(H,24,29)(H,25,26,27)
Molecular weight 418.13 Da
AlogP 3.507900000000001
HBond acceptors 7
HBond donors 3
Atoms 47

References

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    Expert Reviews

    by SERP
    (on 29 May 2016 )
    Cellular Use Rating
    In Model Organisms
    This probe is an allosteric inhibitor of ABL, with sound biochemical and biophysical evidence. However, its effects in cells and animals have primarily been studied in the context of combinations with...
    by SERP
    (on 7 Jun 2016 )
    Cellular Use Rating
    In Model Organisms
    GNF-5 is an allosteric inhibitor of ABL kinase, binding to the myristate pocket near the C-terminus of the kinase domain. GNF-5 in combination with nilotinib is capable of inhibiting phosphorylation...
    by SERP
    (on 4 Nov 2020 )
    Cellular Use Rating
    In Model Organisms
    GNF-5 is an allosteric binder and a clean functional inhibitor of Bcr-Abl in vitro and in cells. The potency of GNF-5 alone is modest and it is best used in combination with an orthosteric inhibitor...
    GNF-5 is an allosteric binder and a clean functional inhibitor or Bcr-Abl in vitro and in cells. The potency of GNF-5 alone is modest and it is best used in combination with an orthosteric inhibitor...
    Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria