GNE-064 | GNE-064 : Inhibitor of BRDs of SMARCA4, SMARCA2, PBRM1 (BD5)
RATINGS:
Cellular Use: (1 reviews)

In Model Organisms: (0 reviews)
Control Compounds
Vendors

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
SMARCA4
  • IC50:35 ± 1 nM
  • Kd:47 nM
  • Kd:10 nM
    SMARCA2
    • Kd:16 nM
    • EC50:100 nM
    PBRM1
    • Kd:18 nM
    • Kd:490 nM
      Inhibitor
      up to 1 uM

      Selectivity

      In Vitro Selectivity Assessment
      Potency Assay Off-Target:
      BromoSCAN of GNE-064 exhibit high potency against SMARCA2, SMARCA4, and PBRM1(5), modest affinity fo ...

      Potency
      Cellular
      In Vitro

      SMARCA4

      Mode of Action: Inhibitor

      Structure-Activity-Relationship data available? No

      DOI Reference: 10.1021/acs.jmedchem.2c00662

      SMARCA2

      Mode of Action: Inhibitor

      Structure-Activity-Relationship data available? No

      DOI Reference: 10.1021/acs.jmedchem.2c00662

      PBRM1

      Mode of Action: Inhibitor

      Structure-Activity-Relationship data available? No

      DOI Reference: 10.1021/acs.jmedchem.2c00662

      In Vivo Validations

      Mouse
      Dose: 0.5 (IV), 1.0 (PO) mg/Kg
      Route of delivery: Intravenous, Oral
      Plasma half life: 1.1 h (IV)
      Systemic clearance: 16 mL/min/Kg
      Cmax: 1.0 uM (PO)
      Bioavailability: 59 %
      Volume of Distribution at Steady-State: 1.0 L/Kg

      DOI Reference: 10.1021/acs.jmedchem.2c00662

      Chemical Information

      Molecular Formula C17H21N5O2
      SMILEs CC(=O)N1CCN(c2cc(-c3ccccc3O)nnc2N)C[C@H]1C
      InChI InChI=1S/C17H21N5O2/c1-11-10-21(7-8-22(11)12(2)23)15-9-14(19-20-17(15)18)13-5-3-4-6-16(13)24/h3-6,9,11,24H,7-8,10H2,1-2H3,(H2,18,20)/t11-/m1/s1
      Molecular weight 327.17 Da
      AlogP 1.4884
      HBond acceptors 7
      HBond donors 3
      Atoms 45

      References

      Cross References

      canSARChEMBLBindingDB

      Expert Reviews


      (on 8 Mar 2026 )
      Cellular Use Rating
      GNE-064 provides a novel tool to study the biology of the family VIII bromodomains of SMARCA2 (BRM) and SMARCA4 (BRG1), and PBRM1 (polybromo-1) bromodomain 5. GNE-064 is potent and selective in biochemical...
      Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria