Chemical Probes logo
FIRSOCOSTAT | FIRSOCOSTAT : Inhibitor of ACACA, ACACB
RATINGS:
Cellular Use: (1 reviews)

In Model Organisms: (1 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
Download

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
ACACA
  • IC50:2.1 nM
  • IC50:66.0 nM (10% serum), 8.7 nM (serum-free)
ACACB
  • IC50:6.1 nM
  • % in:>50% at 1 μM
Inhibitor
up to 1 μM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
>10 μM
Selectivity Assessment Description:
Ricera DrugMatrix Panel (101 receptors, GFs, transporters, and ion channels). Data available in SI o ...

Potency
Cellular
In Vitro

ACACA

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1073/pnas.1520686113

ACACB

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1073/pnas.1520686113

In Vivo Validations

Rat
Dose: 3 mg/kg
Route of delivery: Intravenous
Plasma half life: 4.5 h
Systemic clearance: 33 mL/min/kg
Cmax: 6 μM (plasma), 81 μM (liver), 0.6 μM (quadriceps)
Tmax: 0.25 h
Area Under the Curve:: 1932 ng.h/mL
Fb : 98.6%
Bioavailability: 37% (oral)
Volume of Distribution at Steady-State: 1.9 L/kg
Organ of interest (O): Liver, skeletal muscle

DOI Reference: https://doi.org/10.1073/pnas.1520686113

Negative Control Compounds

ND-022
Notes: hACC1 IC50 3.9 μM; hACC2 IC50 6.6 μM (~1000-fold difference)
ChEMBL ID: CHEMBL4476176

Orthogonal Probes def

MK-4074
Notes: 3 nM potencies for hepatic ACC1/2
ChEMBL ID: CHEMBL4557289

Chemical Information

Molecular Formula C28H31N3O8S
SMILEs COc1ccccc1[C@H](Cn1c(=O)n(C(C)(C)C(=O)O)c(=O)c2c(C)c(-c3ncco3)sc21)OC1CCOCC1
InChI InChI=1S/C28H31N3O8S/c1-16-21-24(32)31(28(2,3)26(33)34)27(35)30(25(21)40-22(16)23-29-11-14-38-23)15-20(39-17-9-12-37-13-10-17)18-7-5-6-8-19(18)36-4/h5-8,11,14,17,20H,9-10,12-13,15H2,1-4H3,(H,33,34)/t20-/m0/s1
Molecular weight 569.18 Da
AlogP 3.9533200000000024
HBond acceptors 11
HBond donors 1
Atoms 71

Expert Reviews

by SERP
(on 28 Feb 2026 )
Cellular Use Rating
In Model Organisms
Progressed to human clinical trials as Firsocostat.
Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria