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ERKi | ERKi : Inhibitor of MAPK1, MAPK3
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (2 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
MAPK1
  • IC50:1.8 nM
  • IC50:4 nM
  • IC50:280 nM
  • IC50:850 nM
  • IC50:91-305
MAPK3
    • IC50:6690 nM
    Inhibitor
    1-5 µM may be more suitable in some cell lines
    1 uM

    Selectivity

    In Vitro Selectivity Assessment
    Selectivity Assessment Description:
    Selectivity within target family: Invitrogen kinase panel. Closest off targets: GSK3A = 0.21 µM, SGK ...
    In Cell Selectivity Assessment
    Selectivity Assessment Description:
    NanoBRET results: GSK3A, SGK1, GSK3B, GRK2 and TBK1 (IC50 > 20µM)

    Potency
    Cellular
    In Vitro

    MAPK1

    Mode of Action: Inhibitor

    Structure-Activity-Relationship data available? No

    DOI Reference: 10.7554/eLife.34311

    MAPK3

    Mode of Action: Inhibitor

    Structure-Activity-Relationship data available? No

    DOI Reference: 10.7554/eLife.34311

    In Vivo Validations

    Rat
    Dose: 1 mg/Kg PO, 0.5 mg/Kg IV
    Route of delivery: Oral, Intravenous
    Plasma half life: 0.4 h
    Systemic clearance: 217 mL/min/kg
    Fb : 84%
    Bioavailability: 13%
    Volume of Distribution at Steady-State: 5.7 L/Kg

    DOI Reference: 10.7554/eLife.34311

    Dog
    Dose: 0.5 mg/Kg PO, 0.25 mg/Kg IV
    Route of delivery: Oral, Intravenous
    Plasma half life: 2.1 h
    Systemic clearance: 89 mL/min/kg
    Fb : 87%
    Bioavailability: 78%
    Volume of Distribution at Steady-State: 15.2 L/Kg

    DOI Reference: 10.7554/eLife.34311

    Negative Control Compounds

    ERKi-NC
    Notes: The negative control is 1000-fold less potent biochemically and shows no activity in cells @ 10µM; GPCR scan shows weak off-target activity for HTR2B (pKi = 6.27).

    Orthogonal Probes def

    SCH772984

    Chemical Information

    Molecular Formula C16H17N5O2
    SMILEs C[C@@H](NC(=O)Nc1cc2[nH]ncc2c(CO)n1)c1ccccc1
    InChI InChI=1S/C16H17N5O2/c1-10(11-5-3-2-4-6-11)18-16(23)20-15-7-13-12(8-17-21-13)14(9-22)19-15/h2-8,10,22H,9H2,1H3,(H,17,21)(H2,18,19,20,23)/t10-/m1/s1
    Molecular weight 311.14 Da
    AlogP 2.3329000000000004
    HBond acceptors 7
    HBond donors 4
    Atoms 40

    Vendors

    Note: This is not an exhaustive list and does not indicate endorsement by the portal.

    Expert Reviews

    by SERP
    (on 2 Feb 2022 )
    Cellular Use Rating
    ...
    by SERP
    (on 16 Feb 2022 )
    Cellular Use Rating
    In Model Organisms
    This is a good additional probe for ERK. As with almost all kinase probes, it's best to also test ERK inhibitors from different scaffolds to ensure any effects are target related.
    by SERP
    (on 7 Aug 2023 )
    Cellular Use Rating
    In Model Organisms
    The assumption is that the probe is ATP competitive. May be used in parallel with orthogonal probe SCH772984. Good selectivity; and reassuring to see that the enantiomer is inactive. Insufficient evidence...
    Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria