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EED226 | EED226 : Allosteric inhibitor of EED
RATINGS:
Cellular Use: (4 reviews)

In Model Organisms: (3 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
EED
  • Kd:82 nM
  • IC50:23.4 nM
Allosteric
100 nM - 1 uM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
EED226 had limited activity against 21 protein methyltransferases (IC50 >100 uM). EED226 inhibited ...
Selectivity Assessment Description:
EED226 had limited activity against 23 kinases (IC50 >10 uM), and 22 GPCRs, ion channels, nucl ...

Potency
Cellular
In Vitro

EED

Mode of Action: Allosteric

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1038/nchembio.2304

In Vivo Validations

Mouse
Dose: 4 and 40 mg/kg
Route of delivery: Oral
Organ of interest (O): Karpas422 xenograft
Target engagement assay: EED226 led to dose-dependent decrease in global H3K27me3 levels.

DOI Reference: 10.1038/nchembio.2304

Orthogonal Probes def

A-395

Chemical Information

Molecular Formula C17H15N5O3S
SMILEs CS(=O)(=O)c1ccc(-c2cnc(NCc3ccco3)n3cnnc23)cc1
InChI InChI=1S/C17H15N5O3S/c1-26(23,24)14-6-4-12(5-7-14)15-10-19-17(22-11-20-21-16(15)22)18-9-13-3-2-8-25-13/h2-8,10-11H,9H2,1H3,(H,18,19)
Molecular weight 369.09 Da
AlogP 2.3999000000000006
HBond acceptors 8
HBond donors 1
Atoms 41

References

Publications

Cross References

Vendors

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Expert Reviews

by SERP
(on 13 Jun 2017 )
Cellular Use Rating
The fact that this compound is non-competitive with respect to SAM and peptide substrate is supported by an X-ray structure and enzymatic competition assays. The furane ring with electron-donating group...
by SERP
(on 14 Jun 2017 )
Cellular Use Rating
In Model Organisms
This allosteric inhibitor of PRC2 competes with the H3K27me3 peptide for binding to EED. So far, it seems to be a very clean inhibitor. The only data on this compound has been published by a single...
by SERP
(on 16 Jun 2017 )
Cellular Use Rating
In Model Organisms
( The reviewer did not leave any comments )
by SERP
(on 29 Aug 2017 )
Cellular Use Rating
In Model Organisms
( The reviewer did not leave any comments )
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