D6-3 | D6-3 : Covalent Inhibitor of CTSL
RATINGS:
Cellular Use: (1 reviews)

In Model Organisms: (1 reviews)
Control Compounds
Vendors

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
CTSL
  • IC50:0.27 nM
  • IC50:260 nM
Covalent Inhibitor
up to 10 uM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
D6-3 inhibitor activity was measured on seven human proteases @ 10 uM. Among them, TMPRSS2, human tr ...
Selectivity Assessment Description:
D6-3 did show inhibition against CatB, CatV, and CatS. Kinact/Ki values of D6–3 for other cathepsins ...

Potency
Cellular
In Vitro

CTSL

Mode of Action: Covalent Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acs.jmedchem.4c00656

In Vivo Validations

Rat
Dose: 10.0 mg/kg
Route of delivery: Intraperitoneal
Plasma half life: 8.5 h
Systemic clearance: 3070 (ml/h/kg)
Cmax: 437 ng/mL
Tmax: 2.33 h
Area Under the Curve:: 2380 (h*ng/mL)

DOI Reference: 10.1021/acs.jmedchem.4c00656

Dose: 25.0 mg/kg
Route of delivery: Oral
Plasma half life: Non-detectable
Systemic clearance: Non-detectable
Cmax: Non-detectable
Tmax: Non-detectable
Area Under the Curve:: Non-detectable

DOI Reference: 10.1021/acs.jmedchem.4c00656

Orthogonal Probes def

canSAR1900166

Chemical Information

Molecular Formula C30H26ClFN2O3
SMILEs O=C(N[C@@H](Cc1ccc(F)cc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)CCl)c1cccc2ccccc12
InChI InChI=1S/C30H26ClFN2O3/c31-19-28(35)26(17-20-7-2-1-3-8-20)33-30(37)27(18-21-13-15-23(32)16-14-21)34-29(36)25-12-6-10-22-9-4-5-11-24(22)25/h1-16,26-27H,17-19H2,(H,33,37)(H,34,36)/t26-,27-/m0/s1
Molecular weight 516.16 Da
AlogP 4.855400000000004
HBond acceptors 5
HBond donors 2
Atoms 63

Expert Reviews


(on 22 Aug 2025 )
Cellular Use Rating
In Model Organisms
The solubility of D6-3 was described as being poor and the formulation for in vivo studies via the i.p route was not disclosed. It is highly recommended that the formulation (s) used by the authors for...
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