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CS640 | CS640 : Inhibitor of CAMK1D
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (2 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
CAMK1D
  • IC50:8 nM
  • IC50:29 nM
Inhibitor
Cellular concentrations of well less than 1 uM should be considered prior to testing at 1 uM
up to 1 uM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
Unselective within the CAMK1 family. CS640 shows excellent selectivity in a Eurofins DiscoverX scree ...
In Cell Selectivity Assessment
Potency Assay Off-Target:
NanoBRET confirms additional CAMK1 family targets: CAMK1B IC50 8.2 nM CAMK1A IC50 23 nM CAMK1G IC50 ...

Potency
Cellular
In Vitro

CAMK1D

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acs.jmedchem.9b01803

In Vivo Validations

Mouse
Dose: 2 mg/Kg
Route of delivery: Intravenous
Plasma half life: 8.3 h
Systemic clearance: 13.7 mL/min/Kg
Cmax: 503 ng/mL
Area Under the Curve:: 2454 ng*h/mL
Volume of Distribution at Steady-State: 7.46 L/Kg

DOI Reference: 10.1021/acs.jmedchem.9b01803

Dose: 10 mg/Kg
Route of delivery: Oral
Plasma half life: 5.9 h
Systemic clearance: 44%
Cmax: 387 ng/mL
Area Under the Curve:: 5408 ng*h/mL

DOI Reference: 10.1021/acs.jmedchem.9b01803

Dose: 20 mg/Kg
Route of delivery: Oral
Plasma half life: 5.9 h
Cmax: 2015 ng/mL
Area Under the Curve:: 28923 ng*h/mL
Bioavailability: 118%

DOI Reference: 10.1021/acs.jmedchem.9b01803

Dose: 40 mg/Kg
Route of delivery: Oral
Plasma half life: 6.2 h
Cmax: 3792 ng/mL
Area Under the Curve:: 49220 ng*h/mL
Bioavailability: 100%

DOI Reference: 10.1021/acs.jmedchem.9b01803

Rat
Dose: 2 mg/Kg
Route of delivery: Intravenous
Plasma half life: 7.7 h
Systemic clearance: 36.5 mL/min/Kg
Cmax: 391 ng/mL
Area Under the Curve:: 913 ng*h/mL
Volume of Distribution at Steady-State: 18.5 L/Kg

DOI Reference: 10.1021/acs.jmedchem.9b01803

Dose: 10 mg/Kg
Route of delivery: Oral
Plasma half life: 7.0 h
Cmax: 199 ng/mL
Area Under the Curve:: 3058 ng*h/mL
Bioavailability: 67.5%

DOI Reference: 10.1021/acs.jmedchem.9b01803

Negative Control Compounds

CS640s
Notes: CS640s was tested in an in house kinase DSF Panel, showing no Tm shift on any kinase.
SMILES: CC(c1nc(C(C)C)cc(Nc2c(C(N)=O)cnc(N3CCC[C@@H](C3)NC)n2)c1)C

Chemical Information

Molecular Formula C21H31N7O
SMILEs CC(C)c1cc(Nc2nc(N3CCC[C@H](N)C3)ncc2C(N)=O)cc(C(C)C)n1
InChI InChI=1S/C21H31N7O/c1-12(2)17-8-15(9-18(26-17)13(3)4)25-20-16(19(23)29)10-24-21(27-20)28-7-5-6-14(22)11-28/h8-10,12-14H,5-7,11,22H2,1-4H3,(H2,23,29)(H,24,25,26,27)/t14-/m0/s1
Molecular weight 397.26 Da
AlogP 2.8884000000000007
HBond acceptors 8
HBond donors 5
Atoms 60

Expert Reviews

by SERP
(on 12 Nov 2023 )
Cellular Use Rating
In Model Organisms
The NanoBRET assay data that was shown in 'In Cell Validation 1' could not be identified in the reference. It might cause some problems that CS640 has the limited selectivity over other CAMK1s.
by SERP
(on 12 Nov 2023 )
Cellular Use Rating
In Model Organisms
The probe CS640 is a highly potent inhibitor of CaMK1D, along with family members CaMK1B, CaMK1A, and CaMK1G (8, 3, 1, and 1 nM respectively). Because it is more potent on the CaMK isoforms than on CaMK1D...
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