CRT0273750 | CRT0273750 : Inhibitor of ENPP2
RATINGS:
Cellular Use: (0 reviews)

In Model Organisms: (1 reviews)
Control Compounds
Vendors

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
ENPP2
  • IC50:1 nM
  • IC50:34 nM
  • EC50:25 nM
Inhibitor
up to 5 uM

Selectivity

In Cell Selectivity Assessment
Potency Assay Off-Target:
IOA-289 was tested in fibrosis screening panels of human primary cells in vitro. Tested at 6 uM, IOA ...
In Vitro Selectivity Assessment
Potency Assay Off-Target:
In vitro safety pharmacology studies showed that IOA-289 has no to minimal potential off-target inte ...
In Vitro Selectivity Assessment
Potency Assay Off-Target:
In the GLP hERG assay, IOA-289 inhibited the hERG tail current with an IC50 of 1.03 μM. Based on the ...

Potency
Cellular
In Vitro

ENPP2

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1016/j.bmcl.2016.10.036

In Vivo Validations

Mouse
Dose: 1 mg/Kg
Route of delivery: Intravenous
Plasma half life: 1.0 h
Systemic clearance: 41 mL/min/Kg
Volume of Distribution at Steady-State: 1.3 L/Kg

DOI Reference: 10.1016/j.bmcl.2016.10.036

Dose: 10 mg/Kg
Route of delivery: Oral
Plasma half life: 1.4 h
Cmax: 3.8 uM
Area Under the Curve:: 3.2 uM*h
Bioavailability: 41%

DOI Reference: 10.1016/j.bmcl.2016.10.036

Rat
Dose: 1 mg/Kg followed after 2 days of washout by a single oral dose of 3 mg/Kg
Route of delivery: Intravenous
Systemic clearance: 19 mL/min/Kg
Fb : 0.02

DOI Reference: 10.1016/j.bmcl.2016.10.036

Chemical Information

Molecular Formula C25H22ClF3N4O2
InChI InChI=1S/C25H22ClF3N4O2/c1-16(18-6-8-19(26)9-7-18)31-23(34)13-12-22-32-21-3-2-14-30-24(21)33(22)15-17-4-10-20(11-5-17)35-25(27,28)29/h2-11,14,16H,12-13,15H2,1H3,(H,31,34)/t16-/m0/s1
Molecular weight 502.14 Da
AlogP 0.0
HBond acceptors 6
HBond donors 1
Atoms 57

References

Cross References

canSARChEMBLBindingDBPDB

Expert Reviews


(on 13 Jul 2025 )
In Model Organisms
This probe is well-validated and was tested in a first-in-human clinical phase I study in healthy volunteers.
Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria