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CFI-402257 | CFI-402257 : ATP competitive inhibitor of TTK
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (2 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
TTK
  • Ki:0.09 ± 0.02 nM
  • IC50:1.7 nM
  • EC50:2 nM
  • EC50:2 nM
  • EC50:4 nM
  • GI50:6,5 nM
Inhibitor
10-100 nM

Selectivity

In Vitro Selectivity Assessment
Selectivity Assessment Description:
Tested against a panel of human kinases at 1 μM: CFI-402257 inhibited none. The molecule is an in ...

Potency
Cellular
In Vitro

TTK

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1073/pnas.1700234114

In Vivo Validations

Rat, Dog, Mouse
Dose: 1 mg/Kg IV, 5 mg/Kg PO
Route of delivery: Intravenous, Oral
Plasma half life: 2-3 h (M)
Systemic clearance: 22 (R), 7.9 (D) mL/min/kg

DOI Reference: 10.1073/pnas.1700234114

Orthogonal Probes def

BAY-1217389
BAY-1161909

Chemical Information

Molecular Formula C28H30N6O3
SMILEs Cc1cc(-c2cnn3c(NC[C@H]4C[C@@](C)(O)C4)cc(Oc4cccnc4)nc23)ccc1C(=O)NC1CC1
InChI InChI=1S/C28H30N6O3/c1-17-10-19(5-8-22(17)27(35)32-20-6-7-20)23-16-31-34-24(30-14-18-12-28(2,36)13-18)11-25(33-26(23)34)37-21-4-3-9-29-15-21/h3-5,8-11,15-16,18,20,30,36H,6-7,12-14H2,1-2H3,(H,32,35)/t18-,28+
Molecular weight 498.24 Da
AlogP 4.357120000000002
HBond acceptors 9
HBond donors 3
Atoms 67

Vendors

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Expert Reviews

by SERP
(on 4 Aug 2021 )
Cellular Use Rating
In Model Organisms
This compound appears to be an excellent probe for inhibiting TTK in cells and in mice. Strong structural characterization and biochemical profiling indicate that it is quite selective toward this kinase....
by SERP
(on 27 Oct 2021 )
Cellular Use Rating
In Model Organisms
CFI-402257 is a selective inhibitor of Monopolar spindle 1 (Mps1; TTK protein kinase). It is an appropriate chemical probe for assessing the utility of TTK as a novel cancer target.
Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria