CCT400028

CCT400028 : Degrader (PROTAC) of AURKA

Structure

SERP Rating

In Cells

(2 ratings)

In Model Organisms

(0 ratings)

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Probe CCT400028 is in the process of SERP review.

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Information

AURKA

Degrader (PROTAC)

up to 1 uM

In Vitro Validations

Uniprot ID: O14965

Target Class: Kinase

Target SubClass: Ser/Thr Kinase

Potency Value: ECmax 620 nM

Potency Assay: Aurora A/PROTAC/CRBN ternary complexes was assessed in the TR- FRET assay

PDB ID for probe-target interaction (3D structure): --

Target aliases:

Aurora kinase A, STK6, STK15, IAK1, BTAK, AYK1, AU ...

DOI Reference: 10.1021/acs.jmedchem.5c03024

In Cell Validations

In Vivo Data

No in Vivo Validations

Off-Target Selectivity Assesments

Potency assay, off target (cells): Proteomics profiling of CCT400028 in Kelly human pediatric neuroblastoma cells treated with 1 μM of compound for 4 h shows only degradation for AURKA.

Potency assay (off target): CCT400028 was profiled in the Eurofins scanEDGE kinase binding assay, which contains 97 kinases distributed across the kinome.

Probe Selectivity in Vitro:

CCT400028 showed excellent binding selectivity against the 97 kinase panel with Aurora A the only kinase that CCT400028 bound to potently (<35% control binding remaining) at 1 μM. Within the Aurora kinase family, CCT400028 showed high selectivity for Aurora A with no measurable affinity for Aurora C and a 28-fold selectivity over Aurora B. CCT400028 would therefore not be expected to affect Aurora B activity in cells due to its weak affinity for Aurora B (2100 nM).

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SERP ratings and comments

SERP Ratings

In Cell Rating

(last updated: 22 Jan 2026 )

SERP+ Ratings

In Cell Rating

SERP+ Comments:

This Aurora Degrader receives full star rating. It is recommended to be used up to 1uM for up to 24 hours. Compound 57 is recommended to be used as negative control in biological assays.

(last updated: 6 Feb 2026 )