CCT400028 | CCT400028 : Degrader (PROTAC) of AURKA
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (0 reviews)
In Vivo
Chemical Information
Vendors

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
AURKA
  • DC50:29 nM
  • Dmax:85%
  • GI50:310 nM
  • Dmax:>95%
Degrader (PROTAC)
up to 1 uM

Selectivity

In Cell Selectivity Assessment
Potency Assay Off-Target:
Proteomics profiling of CCT400028 in Kelly human pediatric neuroblastoma cells treated with 1 μM of ...
In Vitro Selectivity Assessment
Potency Assay Off-Target:
CCT400028 was profiled in the Eurofins scanEDGE kinase binding assay, which contains 97 kinases dist ...
Selectivity Assessment Description:
CCT400028 showed excellent binding selectivity against the 97 kinase panel with Aurora A the only ki ...

Potency
Cellular
In Vitro

AURKA

Mode of Action: Degrader (PROTAC)

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acs.jmedchem.5c03024

Negative Control Compounds

cpd 57
Notes: CRBN-inactive N-methylated version of CCT400028 (57) was unable to reduce Aurora A protein levels at concentrations up to 10 μM in Kelly neuroblastoma cells.
SMILES: CN1C(=O)CCN(C2=CC=C(C=C2)OCCCCCOCCCCCNC(=O)C3=CC=C(C=C3OC)NC4=NC5=C(CN=C(C6=CC(=CC=C65)Cl)C7=C(C=CC=C7OC)F)C=N4)C1=O

Orthogonal Probes def

canSAR7019194
MK-5108

Chemical Information

Coming Soon...

References

Cross References

Expert Reviews


(on 22 Jan 2026 )
Cellular Use Rating
( The reviewer did not leave any comments )
(on 6 Feb 2026 )
Cellular Use Rating
This Aurora Degrader receives full star rating. It is recommended to be used up to 1uM for up to 24 hours. Compound 57 is recommended to be used as negative control in biological assays.
(on 6 Apr 2026 )
Cellular Use Rating
CCT400028 is a PROTAC molecule linking alisertib (Aurora A inhibitor) with phenyl dihydrouracil (CRBN-binding motif) and with greater linker rigidity than PEG linkers.
Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria