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BMS-911543 | BMS-911543 : ATP competitive inhibitor of JAK2
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (3 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
JAK2
  • Ki:0.48 nM
  • IC50:60 nM
  • IC50:61 nM
  • IC50:990 nM
ATP competitive
Up to 200 nM

Selectivity

In Vitro Selectivity Assessment

Potency: IC50 - JAK1 360 nM (Ki=110 nM), JAK3 75 nM (Ki=360 nM), TYK2 66 nM

Potency Assay Off-Target:
Potency was determined using ATP at the Km for each JAK kinase using Caliper LabChip
Selectivity Assessment Description:
Ambit kinome profiling was done against 451 kinases; Targets with measured IC50s in a biochemical ...

Potency
Cellular
In Vitro

JAK2

Mode of Action: ATP competitive

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acsmedchemlett.5b00226

In Vivo Validations

mouse, rat, dog, cyno
Dose: 1-100 mg/kg
Route of delivery: Intravenous, Oral
Plasma half life: mouse/rat 5 h, dog/cyno 2.2-2.4 h
Systemic clearance: 0.55 (mouse), 0.7 (rat), 6.3 (dog), 5.3 (cyno)

DOI Reference: 10.1021/acsmedchemlett.5b00226

Negative Control Compounds

Not available

Orthogonal Probes def

XL019
AZ-960
Gandotinib

Chemical Information

Molecular Formula C23H28N8O
SMILEs CCn1c(C(=O)N(C2CC2)C2CC2)cc2c3c(ncn3C)c(Nc3cc(C)n(C)n3)nc21
InChI InChI=1S/C23H28N8O/c1-5-30-17(23(32)31(14-6-7-14)15-8-9-15)11-16-20-19(24-12-28(20)3)21(26-22(16)30)25-18-10-13(2)29(4)27-18/h10-12,14-15H,5-9H2,1-4H3,(H,25,26,27)
Molecular weight 432.24 Da
AlogP 0.0
HBond acceptors 9
HBond donors 1
Atoms 60
PAINS * Yes

* This is an automated alert only, and may not necessarily indicate an issue with this probe. ( Learn more about PAINS )

References

Publications

Cross References

canSARChEMBLBindingDBPDB

Vendors

Note: This is not an exhaustive list and does not indicate endorsement by the portal.

Expert Reviews

by SERP
(on 3 Aug 2016 )
Cellular Use Rating
In Model Organisms
BMS-911543 is a reversible, pyrrolopyridine ATP-competitive JAK2 inhibitor with high selectivity (JAK2 IC50 =1.1 nM vs JAK1, JAK3, Tyk2 IC50 = 360, 75, 66 nM respectively and SET2 IC50 = 60 nM). In...
by SERP
(on 18 Jan 2017 )
Cellular Use Rating
In Model Organisms
BMS-911543 has a favorable profile in an in vitro panel of 45 targets, wherein no activity (IC50 >25 uM) was observed for all targets except PDE4 (IC50: 5.6 uM). No HepG2 cyctotoxicity is observed....
by SERP
(on 28 Jun 2017 )
Cellular Use Rating
In Model Organisms
High selectivity for JAK2 over all the other family members (JAK1, JAK3 and TYK2) differentiates this compound from other JAK inhibitors. Overall kinase selectivity is excellent, and a full KinomeScan...
Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria