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BI-9321 | BI-9321 : Antagonist of NSD3
RATINGS:
Cellular Use: (1 reviews)

In Model Organisms: (0 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
NSD3 (Other variant:PWWP1 domain)
  • Kd:166 ± 3 nM
  • Kd:445 ± 8 nM
  • ΔTm:5.5 °C
  • IC50:203 nM
  • IC50:1400 ± 500nM
  • IC50:1200 ± 60 nM
  • INH:1000 nM
Antagonist
up to 10 uM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
BI-9321 was profiled against 14 other purified PWWP domains using DSF. No stabilization ≥2 °C was ob ...
In Cell Selectivity Assessment
Potency Assay Off-Target:
Selectivity profiling by quantitative chemical proteomics revealed NSD3 as interactor of immobilized ...

Potency
Cellular
In Vitro

NSD3 (Other variant:PWWP1 domain)

Mode of Action: Antagonist

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1038/s41589-019-0310-x

Negative Control Compounds

BI-9466
Notes: IC50 = 120 µM; inactive in cell assays
SMILES: CC1=CC(CN)=CC(C)=C1C2=C(C3=CN=CN3C)N(C)C=N2

Chemical Information

Molecular Formula C22H21FN4
SMILEs Cc1cc(CN)cc(C)c1-c1ncn(C)c1-c1ccnc2cc(F)ccc12
InChI InChI=1S/C22H21FN4/c1-13-8-15(11-24)9-14(2)20(13)21-22(27(3)12-26-21)18-6-7-25-19-10-16(23)4-5-17(18)19/h4-10,12H,11,24H2,1-3H3
Molecular weight 360.18 Da
AlogP 4.5169400000000035
HBond acceptors 4
HBond donors 2
Atoms 48

Vendors

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Expert Reviews

by SERP
(on 3 Feb 2023 )
Cellular Use Rating
This probe is specifically described as a high quality tool to study PWWP1-dependent interactions of NSD3 in cells.
Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria