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BI-5521 | BI-5521 : ATP competitive of GSK3A, GSK3B
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (2 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
GSK3A
  • IC50:2 nM
  • EC50:3 nM
GSK3B
  • IC50:5 nM
    ATP competitive
    up to 100 nM

    Selectivity

    In Vitro Selectivity Assessment
    DYRK1A

    Gene ID: Q13627

    Organism: Homo sapiens

    Family: CMGC Ser/Thr protein kinase family

    Potency: % in - 99% at 500 nM

    Potency Assay Off-Target:
    BI-5521 is similarly active on both isoforms GSK-3α and GSK-3β. Selectivity against other protein ki ...

    Potency
    Cellular
    In Vitro

    GSK3A

    Mode of Action: ATP competitive

    Structure-Activity-Relationship data available? Yes

    DOI Reference: 10.1002/cmdc.202300031

    GSK3B

    Mode of Action: ATP competitive

    Structure-Activity-Relationship data available? No

    DOI Reference: 10.1002/cmdc.202300031

    In Vivo Validations

    Rat
    Dose: 4.20 mg/kg IV, 0.42 mg/kg PO
    Route of delivery: Intravenous, Oral
    Plasma half life: MRT 2.90 h IV
    Systemic clearance: 32% QH IV
    Cmax: 15.5 nM PO
    Tmax: 4 h PO
    Area Under the Curve:: 519 nM*h IV
    Bioavailability: 17%
    Volume of Distribution at Steady-State: 5.6 L/Kg IV

    DOI Reference: 10.1002/cmdc.202300031

    Negative Control Compounds

    BI-4481
    Notes: BI-4481 (cpd 44), serves as a negative control with an IC50 against GSK-3β of >10,000 nM
    SMILES: CCN1C(=O)\C(=C(/NC2CCN(C)CC2)C2=CC3=C(OCO3)C=C2)C2=C1C=CC(=C2)C(C)=O

    Orthogonal Probes def

    AZD-1080
    LY2090314
    TIDEGLUSIB

    Chemical Information

    Molecular Formula C24H25N3O4
    SMILEs CC(=O)c1ccc2c(c1)/C(=C(/NC1CCN(C)CC1)c1ccc3c(c1)OCO3)C(=O)N2
    InChI InChI=1S/C24H25N3O4/c1-14(28)15-3-5-19-18(11-15)22(24(29)26-19)23(25-17-7-9-27(2)10-8-17)16-4-6-20-21(12-16)31-13-30-20/h3-6,11-12,17,25H,7-10,13H2,1-2H3,(H,26,29)/b23-22-
    Molecular weight 419.18 Da
    AlogP 0.0
    HBond acceptors 7
    HBond donors 2
    Atoms 56

    Expert Reviews

    by SERP
    (on 14 Jul 2024 )
    Cellular Use Rating
    In Model Organisms
    BI-5521 is a highly potent (nanomolar range) and selective GSK-3a and GSK-3b ATP-competitive inhibitor, with similar potencies for the 2 isoforms. Both in vitro (radioligand binding assay) and cell activity...
    by SERP
    (on 7 Aug 2024 )
    Cellular Use Rating
    In Model Organisms
    BI-5521 is a potent dual inhibitor of GSK-3a and GSK-3b with biochemical potencies of 2 and 1 nM respectively against these two targets. It is a very potent inhibitor of DYRK1A, showing 99% inhibition...
    Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria