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BI-2545 | Inhibitor of ENPP2
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (3 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
ENPP2
  • IC50:2.2 nM (human); 3.4 nM (rat)
  • IC50:29 nM (human) ; 96 nM (rat)
Inhibitor
1 uM

Selectivity

In Cell Selectivity Assessment
Selectivity Assessment Description:
At 10 µM (CEREP): 4/48 off targets >50% (5-HT2A, Ca2+ channel, Na+ channel, norepinephrine transport ...

Potency
Cellular
In Vitro

ENPP2

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? No

In Vivo Validations

Rat
Dose: 10 mg/Kg PO, 1 mg/Kg IV
Route of delivery: Intravenous, Oral
Plasma half life: 3.4 h
Systemic clearance: 7 ml/(min*Kg)

Reference: --

Negative Control Compounds

BI-3017

Orthogonal Probes def

PF-8380

Chemical Information

Molecular Formula C23H19F6N5O3
SMILEs O=C(NC[C@H]1[C@@H]2CN(C(=O)OCc3cc(C(F)(F)F)cc(C(F)(F)F)c3)C[C@H]12)c1ccc2[nH]nnc2c1
InChI InChI=1S/C23H19F6N5O3/c24-22(25,26)13-3-11(4-14(6-13)23(27,28)29)10-37-21(36)34-8-16-15(17(16)9-34)7-30-20(35)12-1-2-18-19(5-12)32-33-31-18/h1-6,15-17H,7-10H2,(H,30,35)(H,31,32,33)/t15-,16-,17+
Molecular weight 527.14 Da
AlogP 4.0126
HBond acceptors 8
HBond donors 2
Atoms 56

Vendors

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Expert Reviews

by SERP
(on 28 Jun 2020 )
Cellular Use Rating
In Model Organisms
BI-2545 represents a suitable chemical probe for ENPP2. While of a similar chemical class to PF-8380, BI-2545 is more potent in whole blood experiments and achieved high in vivo potency when administered...
by SERP
(on 28 Jun 2020 )
Cellular Use Rating
In Model Organisms
In the original article, the compound is dissolved in 0.5% Natrosol For p.o. admistration. The compound can also be administered i.v. by dissolving in 7% HP-β-CD.
by SERP
(on 28 Jun 2020 )
Cellular Use Rating
In Model Organisms
The benzotriazole BI-2545 compound is a potent inhibitor of human Autotaxin (ATX) both in cells (IC50 of 29 nM) and in vivo (IC50 of 96 nM in rodents). ATX is an enzyme that controls levels of...
Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria