BAY-8400 | BAY-8400 : Inhibitor of PRKDC
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (2 reviews)
Control Compounds

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
PRKDC
  • IC50:81 nM
  • IC50:69 nM
Inhibitor
500 nM
up to 1 uM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
Selectivity vs ATM (ratio of biochemical IC50 values) >200. Evaluation of BAY-8400 in the in-house k ...
In Cell Selectivity Assessment
Potency Assay Off-Target:
BAY-8400 showed good selectivity against PI3K and ATR (ratio of IC50 values: PI3K/DNA-PK > 15 and AT ...

Potency
Cellular
In Vitro

PRKDC

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acs.jmedchem.1c00762

In Vivo Validations

Rat
Dose: 0.3 mg/kg IV, 0.6 mg/kg PO
Route of delivery: Intravenous, Oral
Plasma half life: 0.84 h IV
Systemic clearance: 2.2 L/h/Kg IV
Bioavailability: 22% PO
Volume of Distribution at Steady-State: 1.8 L/Kg IV

DOI Reference: 10.1021/acs.jmedchem.1c00762

Mouse
Dose: 0.3 mg/kg
Route of delivery: Intravenous
Plasma half life: 0.68 h IV
Systemic clearance: 8.1 L/h/kg
Volume of Distribution at Steady-State: 3.7 L/kg

DOI Reference: 10.1021/acs.jmedchem.1c00762

Chemical Information

Molecular Formula C21H17F2N5O
SMILEs FC(F)c1cncc(-c2ccc3ncc4nnc(C5=CCCOCC5)n4c3c2)c1
InChI InChI=1S/C21H17F2N5O/c22-20(23)16-8-15(10-24-11-16)14-3-4-17-18(9-14)28-19(12-25-17)26-27-21(28)13-2-1-6-29-7-5-13/h2-4,8-12,20H,1,5-7H2
Molecular weight 393.14 Da
AlogP 0.0
HBond acceptors 6
HBond donors --
Atoms 46

References

Cross References

canSARChEMBLBindingDB

Vendors

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Expert Reviews


(on 29 Jan 2024 )
Cellular Use Rating
In Model Organisms
BAY-8400 is only a weakly active and selective inhibitor of DNA-PK, compared to AZD7648. The small difference in antiproliferative activity of BAY-8400 in the absence and presence of DSB-inducing challenges...
(on 20 Feb 2024 )
Cellular Use Rating
In Model Organisms
The compound BAY-8400, represents an additional alternative compound for a selective DNA-PK inhibitor, besides the inhibitor AZD7648. However, it should be taken into account that the compound BAY-8400...
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