BAY-6035 | BAY-6035 : apeptide-competitive inhibitor of SMYD3
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (0 reviews)
In Vivo
Vendors

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
SMYD3
  • ΔTm:8.34 K
  • IC50:88 nM
  • Kd:133 nM
  • Kd:97 nM
  • IC50:183 ± 13 nM
peptide-competitive
5-10µM could be used as no toxicity has been observed up to 20 µM
1 uM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
Selectivity was assessed against a set of 34 protein methyltransferases, including several that cont ...
In Cell Selectivity Assessment
Potency Assay Off-Target:
BAY-6035 was used to explore if inhibition of SMYD3 leads to inhibition of proliferation in 13 selec ...

Potency
Cellular
In Vitro

SMYD3

Mode of Action: peptide-competitive

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1177/24725552211019409

Negative Control Compounds

BAY-444
Notes: BAY-444 inhibits the in vitro methylation of MEKK2 with IC50 = 23 micromolar.
SMILES: C[C@@H]1CC(NC2=CC(C(NCCC3CC3)=O)=CC=C2N1C(N4CC5CC5C4)=O)=O

Chemical Information

Molecular Formula C22H28N4O3
SMILEs C[C@H]1CC(=O)Nc2cc(C(=O)NCCC3CC3)ccc2N1C(=O)N1CC2CC2C1
InChI InChI=1S/C22H28N4O3/c1-13-8-20(27)24-18-10-15(21(28)23-7-6-14-2-3-14)4-5-19(18)26(13)22(29)25-11-16-9-17(16)12-25/h4-5,10,13-14,16-17H,2-3,6-9,11-12H2,1H3,(H,23,28)(H,24,27)/t13-,16?,17?/m0/s1
Molecular weight 396.22 Da
AlogP 0.0
HBond acceptors 7
HBond donors 2
Atoms 57

Expert Reviews


(on 14 Feb 2023 )
Cellular Use Rating
( The reviewer did not leave any comments )
(on 21 Feb 2023 )
Cellular Use Rating
This is a well validated probe molecule which surpasses many of the SGC set criteria for potency and selectivity, and a very weak binding control compound is included. It has been through several key off...
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