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BAY-299 | Antagonist of BRD1, TAF1, TAF1L
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (3 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
BRD1
  • IC 50:67 nM
  • IC 50:575 nM H4, 825 nM H3.3
TAF1
  • IC 50:8 nM (BD2)
  • IC 50:970 nM H4, 1,400 nM H3.3
TAF1L
  • IC 50:106 nM (BD2)
    Antagonist
    2-500 nM

    Selectivity

    In Vitro Selectivity Assessment

    Potency: Kd - 1,390 nM

    Potency Assay Off-Target:
    Thermal Shift Assay on a panel of 48 bromodomains and BROMOscan panel.
    Selectivity Assessment Description:
    LeadProfilingScreen (Cerep) containing 68 potential protein targets at 10 uM, with no inhibition or ...
    In Cell Selectivity Assessment
    Potency Assay Off-Target:
    NanoBRET
    Selectivity Assessment Description:
    BAY-299 showed no activity against BRD4-H4 or BRPF1-H4 in NanoBRET assays up to 10 uM.

    Potency
    Cellular
    In Vitro

    BRD1

    Mode of Action: Antagonist

    Structure-Activity-Relationship data available? Yes

    DOI Reference: 10.1021/acs.jmedchem.7b00306

    TAF1

    Mode of Action: Antagonist

    Structure-Activity-Relationship data available? Yes

    DOI Reference: 10.1021/acs.jmedchem.7b00306

    TAF1L

    Mode of Action: Antagonist

    Structure-Activity-Relationship data available? Yes

    DOI Reference: 10.1021/acs.jmedchem.7b00306

    In Vivo Validations

    Rat
    Dose:
    Route of delivery: Intravenous, Other, Intragastrally
    Plasma half life: 10 h (IV)
    Systemic clearance: 0.73 L/h/kg (IV)

    DOI Reference: 10.1021/acs.jmedchem.7b00306

    Negative Control Compounds

    BAY-364

    Orthogonal Probes def

    NI-57 (inhibits BRPF family)
    OF-1 (inhibits BRPF family)

    Chemical Information

    Molecular Formula C25H23N3O4
    SMILEs Cc1cc2c(cc1N1C(=O)c3cccc4c(CCCO)ccc(c34)C1=O)n(C)c(=O)n2C
    InChI InChI=1S/C25H23N3O4/c1-14-12-20-21(27(3)25(32)26(20)2)13-19(14)28-23(30)17-8-4-7-16-15(6-5-11-29)9-10-18(22(16)17)24(28)31/h4,7-10,12-13,29H,5-6,11H2,1-3H3
    Molecular weight 429.17 Da
    AlogP 0.0
    HBond acceptors 7
    HBond donors 1
    Atoms 55

    References

    Publications

    Cross References

    canSARChEMBLBindingDBPDBPubMed

    Vendors

    Note: This is not an exhaustive list and does not indicate endorsement by the portal.

    Expert Reviews

    by SERP
    (on 21 Jun 2017 )
    Cellular Use Rating
    In Model Organisms
    As the compound showed similar binding affinity between two type of Bromo domains (BDs) from two proteins with very different roles in transcriptional regulation means that it is not amenable for use...
    by SERP
    (on 30 Jun 2017 )
    Cellular Use Rating
    In Model Organisms
    Cellular data show activity of BAY-299 on BRPF2 BD/H3.3, BRPF2 BD/H4, TAF1 BD2/H3.3, and TAF1 BD2/H4. However, little or no anti-proliferative affect was detected.
    by SERP
    (on 28 Jul 2017 )
    Cellular Use Rating
    In Model Organisms
    Because the compound showed similar binding affinity for two types of Bromodomains (BDs) from two proteins with very different roles in transcriptional regulation, it is not amenable for use as a chemical...
    Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria