AZD2014 | AZD2014 : ATP competitive inhibitor of MTOR
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (3 reviews)
Control Compounds

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
MTOR
  • IC50:2.8 nM
  • IC50:80 nM
ATP competitive
10 nM - 2 uM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
The activity of the lipid kinases, class I PI3Ks α, β, γ, δ was measured with the lipid PIP2 as subs ...

Potency
Cellular
In Vitro

MTOR

Mode of Action: ATP competitive

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1158/1535-7163.MCT-15-0365

In Vivo Validations

Rat, Mouse
Dose: 4 uM/kg (rat), 7.5-15 mg/kg (mouse)
Route of delivery: Intravenous, Oral
Systemic clearance: 12.6 mL/min/kg (rat), 10.4 (mouse)
Organ of interest (O): MCF7 xenograft
Target engagement assay: In xenografts, AZD2014 dose-dependently decrease protein-substrate phosphorylation (S6 and AKT).

DOI Reference: 10.1158/1535-7163.MCT-15-0365

Orthogonal Probes def

Rapamycin
eCF309

Chemical Information

Molecular Formula C25H30N6O3
SMILEs CNC(=O)c1cccc(-c2ccc3c(N4CCOC[C@@H]4C)nc(N4CCOC[C@@H]4C)nc3n2)c1
InChI InChI=1S/C25H30N6O3/c1-16-14-33-11-9-30(16)23-20-7-8-21(18-5-4-6-19(13-18)24(32)26-3)27-22(20)28-25(29-23)31-10-12-34-15-17(31)2/h4-8,13,16-17H,9-12,14-15H2,1-3H3,(H,26,32)/t16-,17-/m0/s1
Molecular weight 462.24 Da
AlogP 0.0
HBond acceptors 9
HBond donors 1
Atoms 64

References

Vendors

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Expert Reviews


(on 27 May 2016 )
Cellular Use Rating
In Model Organisms
( The reviewer did not leave any comments )
(on 1 Jun 2016 )
Cellular Use Rating
In Model Organisms
Selectivity over 200 typical protein kinases and PI3Ks is >1000X. However, selectivity over atypical lipid-like protein kinases (e.g., ATM, ATR, DNA-PK) does not appear to have been disclosed, and this...
(on 5 Jan 2017 )
Cellular Use Rating
In Model Organisms
( The reviewer did not leave any comments )
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