ART5537 | ART5537 : Inhibitor of EXO1
RATINGS:
Cellular Use: (1 reviews)

In Model Organisms: (0 reviews)
Chemical Information
Vendors

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
EXO1
  • IC50:3.4 nM
  • Kd:6.8 nM
  • EC50:55 nM
  • EC50:7.2 nM
  • Activity:1 µM
Inhibitor
1µM

Selectivity

In Cell Selectivity Assessment
Potency Assay Off-Target:
ART5537 was found not to be broadly cytotoxic in a panel of 93 cancer cell lines, or in two addition ...
In Vitro Selectivity Assessment
Potency Assay Off-Target:
The broader nuclease selectivity profile of ART5537 is also supportive of EXO1-driven phenotypes, wi ...

Potency
Cellular
In Vitro

EXO1

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acs.jmedchem.5c02593

In Vivo Validations

Mouse
Dose: 0.2 mg/Kg
Route of delivery: Intravenous
Plasma half life: 0.7 h
Systemic clearance: 111 mL/min/kg
Volume of Distribution at Steady-State: 5.7 L/Kg

DOI Reference: 10.1021/acs.jmedchem.5c02593

Negative Control Compounds

cpd 30
Notes: Tested in biochemical and cell assay, cpd 30 shows a reduced inhibitory activity (IC50 344 nM Biochemical, Kd 174 nM SPR, EC50 396 nM HR)
SMILES: C[C@@H](N1CN(N2C=CC(=O)C(=C2C1=O)O)c1ncc(c2ccncc12)Cl)c1ccccc1

Chemical Information

Coming Soon...

References

Cross References

Expert Reviews


(on 1 Jan 2026 )
Cellular Use Rating
ART5537 is a promising and highly selective chemical probe for EXO1, demonstrating excellent performance in biochemical and cellular assays. However, its current pharmacokinetic limitations (high clearance,...
Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria