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Adavosertib | Adavosertib : ATP competitive inhibitor of WEE1
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (2 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
WEE1
  • IC50:5.2 nM
  • EC50:49 nM (alone); 85 nM (in combination
ATP competitive
300-1000 nM
30-100 nM or 300 nM (alone)

Selectivity

In Vitro Selectivity Assessment
Selectivity Assessment Description:
Among 223 kinases in the Upstate Kinase Profiler panel, only 8 kinases were inhibited by >80% @ 1 μM ...

Potency
Cellular
In Vitro

WEE1

Mode of Action: ATP competitive

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1158/1535-7163.MCT-09-0463

In Vivo Validations

nude rats bearing WiDr (human colorectal) tumors
Dose: 5, 10, or 20 mg/kg, Reviewer suggest: 20 and 30 mg/Kg
Route of delivery: Oral

DOI Reference: 10.1158/1535-7163.MCT-09-0463

Chemical Information

Molecular Formula C27H32N8O2
SMILEs C=CCn1c(=O)c2cnc(Nc3ccc(N4CCN(C)CC4)cc3)nc2n1-c1cccc(C(C)(C)O)n1
InChI InChI=1S/C27H32N8O2/c1-5-13-34-25(36)21-18-28-26(29-19-9-11-20(12-10-19)33-16-14-32(4)15-17-33)31-24(21)35(34)23-8-6-7-22(30-23)27(2,3)37/h5-12,18,37H,1,13-17H2,2-4H3,(H,28,29,31)
Molecular weight 500.26 Da
AlogP 2.886
HBond acceptors 10
HBond donors 2
Atoms 69
PAINS * Yes

* This is an automated alert only, and may not necessarily indicate an issue with this probe. ( Learn more about PAINS )

Vendors

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Expert Reviews

by SERP
(on 10 Dec 2020 )
Cellular Use Rating
In Model Organisms
MK-1775 (Adavosertib) is a potent inhibitor of Wee1 and Wee2, with off target biochemical and cellular activity on a number of other kinases, including polo-like kinase-1 (PLK1).
by SERP
(on 21 Dec 2020 )
Cellular Use Rating
In Model Organisms
Adavosertib unarguably displays both potent WEE1 kinase affinity and a strong phenotype when in combination with common chemotherapies in vivo. However there are studies since published (10.1021/acschembio.7b00147,...
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