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ABPA3 | ABPA3 : Covalent Inhibitor of UBA1, UBA3
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (2 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
UBA1
  • IC 50:43 nM
  • IC 50:2500 nM
UBA3
  • IC 50:43 nM
  • IC 50:80 nM
Covalent Inhibitor
3-5 uM for 16h

Selectivity

In Vitro Selectivity Assessment
Selectivity Assessment Description:
ABP A3 is an AMP mimic and therefore can inhibit protein kinases. Its close analogue MLN4924 was pro ...
In Cell Selectivity Assessment
Potency Assay Off-Target:
Cells were treated with ABP A3 and covalent ABP A3-Ubl adducts were visualized using in gel scanning ...
Selectivity Assessment Description:
We also evaluated if ABP A3 inhibits closely related analogues SUMO E1, ISG15 E1, Ufm1 E1. We did no ...

Potency
Cellular
In Vitro

UBA1

Mode of Action: Covalent Inhibitor

Structure-Activity-Relationship data available? No

DOI Reference: 10.1039/C5SC01351H

UBA3

Mode of Action: Covalent Inhibitor

Structure-Activity-Relationship data available? No

DOI Reference: 10.1039/C5SC01351H

Negative Control Compounds

N/A

Orthogonal Probes def

MLN7243
TAK-243
Pevonedistat

Chemical Information

Molecular Formula C18H18N6O6S
SMILEs C#Cc1cccc(Nc2ncnc3c2ncn3[C@@H]2O[C@H](COS(N)(=O)=O)[C@@H](O)[C@H]2O)c1
InChI InChI=1S/C18H18N6O6S/c1-2-10-4-3-5-11(6-10)23-16-13-17(21-8-20-16)24(9-22-13)18-15(26)14(25)12(30-18)7-29-31(19,27)28/h1,3-6,8-9,12,14-15,18,25-26H,7H2,(H2,19,27,28)(H,20,21,23)/t12-,14-,15-,18-/m1/s1
Molecular weight 446.10 Da
AlogP -0.6094999999999993
HBond acceptors 12
HBond donors 5
Atoms 49

References

Publications

Cross References

Expert Reviews

by SERP
(on 6 Sept 2016 )
Cellular Use Rating
ABPA3 is an activity-based probe that covalently inhibits the ubiquitin- and Nedd8-activating E1 enzymes (UBA1/UBA3), resulting in inhibition of both Nedd8-dependent and Nedd8-independent ubiquitin...
by SERP
(on 27 Dec 2016 )
Cellular Use Rating
In Model Organisms
The published characterization of ABPA3 rests largely on phenotypic, cell-based proteomics in A549 cells. The results, showing selective formation of covalent complexes between ABPA3 and ubiquitin and...
by SERP
(on 13 Jul 2017 )
Cellular Use Rating
In Model Organisms
This compound is a non-specific, covalent inhibitor of ubiquitinylation and Nedd8 and should therefore be used with caution as a single agent, as this polypharmacolgy will almost certainly confound...
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