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A-1596586 | A-1596586 : Corrector of CFTR
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (0 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
CFTR
    • EC50:28 nM
    • EC50:130 nM
    • EC50:35 nM
    Corrector
    up to 1 uM

    Selectivity

    In Vitro Selectivity Assessment
    Selectivity Assessment Description:
    Selectivity outside target family: Closest off targets at 10 µM are BZD peripheral (95.5 %), ADORA3 ...

    Potency
    Cellular
    In Vitro

    CFTR

    Mode of Action: Corrector

    Structure-Activity-Relationship data available? Yes

    DOI Reference: 10.1021/acs.jmedchem.7b01339

    Negative Control Compounds

    A-1596584
    Notes: Negative control A-1596584 is ~ 8 fold less potent in restoring CFTR surface expression and > 100 fold less potent in restoring CFTR function. Note: The negative control binds the target but doesn’t inhibit the function; it is not functional. The control at 10 µM inhibits TACR1 (92.1 %), Cl- channel (GABA gated) (82.4 %), BZD peripheral (79.1%), CNR2 (73.7 %), ADORA3 (67.7%). Closest hits in the GPCR scan (43) are GABA/PBR (Ki = 217.72 nM), TMEM97 (Ki =583.98 nM) and SIGMAR1 (Ki =2740.31 nM).

    Chemical Information

    Molecular Formula C29H27F2NO7
    SMILEs COc1ccc2c(c1)O[C@@H](c1ccc(OC)c(OC)c1)C[C@H]2NC(=O)C1(c2ccc3c(c2)OC(F)(F)O3)CC1
    InChI InChI=1S/C29H27F2NO7/c1-34-18-6-7-19-20(15-23(37-24(19)14-18)16-4-8-21(35-2)25(12-16)36-3)32-27(33)28(10-11-28)17-5-9-22-26(13-17)39-29(30,31)38-22/h4-9,12-14,20,23H,10-11,15H2,1-3H3,(H,32,33)/t20-,23-/m1/s1
    Molecular weight 539.18 Da
    AlogP 5.4468
    HBond acceptors 8
    HBond donors 1
    Atoms 66

    Vendors

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    Expert Reviews

    by SERP
    (on 25 Feb 2026 )
    Cellular Use Rating
    The probe is suitable for in vitro testing but there are other molecules from the series, carboxylic acids (see Table 4 from DOI: 10.1021/acs.jmedchem.7b01339) which are more potent, less lipophilic, and...
    by SERP
    (on 18 Mar 2026 )
    Cellular Use Rating
    I cannot support this candidate drug as a selective probe for CFTR as there is no data at hand demonstrating the compound acts directly on the target. What is good is the description of a clear SAR, but...
    Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria