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A-1596586 | A-1596586 : Corrector of CFTR
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (0 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
CFTR
    • EC50:28 nM
    • EC50:130 nM
    • EC50:35 nM
    Corrector
    up to 1 uM

    Selectivity

    In Vitro Selectivity Assessment
    Selectivity Assessment Description:
    Selectivity outside target family: Closest off targets at 10 µM are BZD peripheral (95.5 %), ADORA3 ...

    Potency
    Cellular
    In Vitro

    CFTR

    Mode of Action: Corrector

    Structure-Activity-Relationship data available? Yes

    DOI Reference: 10.1021/acs.jmedchem.7b01339

    Negative Control Compounds

    A-1596584
    Notes: Negative control A-1596584 is ~ 8 fold less potent in restoring CFTR surface expression and > 100 fold less potent in restoring CFTR function. Note: The negative control binds the target but doesn’t inhibit the function; it is not functional. The control at 10 µM inhibits TACR1 (92.1 %), Cl- channel (GABA gated) (82.4 %), BZD peripheral (79.1%), CNR2 (73.7 %), ADORA3 (67.7%). Closest hits in the GPCR scan (43) are GABA/PBR (Ki = 217.72 nM), TMEM97 (Ki =583.98 nM) and SIGMAR1 (Ki =2740.31 nM).

    Chemical Information

    Molecular Formula C29H27F2NO7
    SMILEs COc1ccc2c(c1)O[C@@H](c1ccc(OC)c(OC)c1)C[C@H]2NC(=O)C1(c2ccc3c(c2)OC(F)(F)O3)CC1
    InChI InChI=1S/C29H27F2NO7/c1-34-18-6-7-19-20(15-23(37-24(19)14-18)16-4-8-21(35-2)25(12-16)36-3)32-27(33)28(10-11-28)17-5-9-22-26(13-17)39-29(30,31)38-22/h4-9,12-14,20,23H,10-11,15H2,1-3H3,(H,32,33)/t20-,23-/m1/s1
    Molecular weight 539.18 Da
    AlogP 5.4468
    HBond acceptors 8
    HBond donors 1
    Atoms 66

    Vendors

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    Expert Reviews

    by SERP
    (on 25 Feb 2026)
    Cellular Use Rating
    The probe is suitable for in vitro testing but there are other molecules from the series, carboxylic acids (see Table 4 from DOI: 10.1021/acs.jmedchem.7b01339) which are more potent, less lipophilic, and...
    by SERP
    (on 18 Mar 2026)
    Cellular Use Rating
    I cannot support this candidate drug as a selective probe for CFTR as there is no data at hand demonstrating the compound acts directly on the target. What is good is the description of a clear SAR, but...
    Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria