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25CN-NBOH | Agonist of HTR2A, HTR2C, HTR2B
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (3 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
HTR2A
  • Ki:0.81 nM
  • EC 50:2.4 nM
HTR2C
  • Ki:42 nM (2C)
  • EC 50:350 nM
HTR2B
  • Ki:30 nM
    Agonist
    up to 500 nM

    Selectivity

    In Vitro Selectivity Assessment
    Potency Assay Off-Target:
    Broad profiling screens performed at Eurofins and PDSP
    Selectivity Assessment Description:
    25CN-NBOH also displayed appreciable binding affinity at 5-HT6 (Ki value of 310 nM), dopamine D4 (Ki ...
    In Vitro Selectivity Assessment
    Selectivity Assessment Description:
    Tested at a wide range of kinases and other enzymes in enzymatic assays, displaying no significant a ...

    Potency
    Cellular
    In Vitro

    HTR2A

    Mode of Action: Agonist

    Structure-Activity-Relationship data available? Yes

    DOI Reference: 10.1021/cn400216u

    HTR2C

    Mode of Action: Agonist

    Structure-Activity-Relationship data available? Yes

    DOI Reference: 10.1021/cn400216u

    HTR2B

    Mode of Action: Agonist

    Structure-Activity-Relationship data available? Yes

    DOI Reference: 10.1021/cn400216u

    In Vivo Validations

    Mouse
    Dose: 0.1 to 10 mg/Kg
    Route of delivery: Intraperitoneal, Subcutaneous
    Organ of interest (O): Brain

    DOI Reference: 10.1021/cn400216u

    Chemical Information

    Molecular Formula C18H20N2O3
    SMILEs COc1cc(CCNCc2ccccc2O)c(OC)cc1C#N
    InChI InChI=1S/C18H20N2O3/c1-22-17-10-15(11-19)18(23-2)9-13(17)7-8-20-12-14-5-3-4-6-16(14)21/h3-6,9-10,20-21H,7-8,12H2,1-2H3
    Molecular weight 312.15 Da
    AlogP 2.613380000000001
    HBond acceptors 5
    HBond donors 2
    Atoms 43
    PAINS * Yes

    * This is an automated alert only, and may not necessarily indicate an issue with this probe. ( Learn more about PAINS )

    Vendors

    Note: This is not an exhaustive list and does not indicate endorsement by the portal.

    Expert Reviews

    by SERP
    (on 25 Jul 2020)
    Cellular Use Rating
    In Model Organisms
    Need to be aware compound is active on multiple targets - HTR2A, HTR2B, and HTR2C - when drawing biological conclusions from studies carried out with this compound. Important to measure drug concentrations...
    by SERP
    (on 4 Aug 2020)
    Cellular Use Rating
    In Model Organisms
    (The reviewer did not leave any public comments)
    by SERP
    (on 13 Aug 2020)
    Cellular Use Rating
    In Model Organisms
    Mode of in vivo delivery i.p or s.c. @ 1.5 mg/kg is indicated by Buchborn 2018. Note potential for rapid desensitization in mouse models
    Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria